Flagellin Protein (FliC) (AA 1-495) (His tag)
-
- 抗原 See all Flagellin (FliC) 蛋白
- Flagellin (FliC)
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-495
-
宿主
- 人, 小鼠
-
资源
- 大肠杆菌(E. Coli)
- 标记
- This Flagellin protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 特异性
- Detected by human and mouse TLR5 (Toll-like receptor 5) and mouse NLRC4 (Nod-like receptor [NLR] family caspase activation and recruitment domain [CARD] domain-containing protein 4).
- 交叉反应
- 人, 小鼠
- 产品特性
- The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) is fused at the N-terminus to a His-tag.
- 纯度
- >95 % (SDS-PAGE)
- 内毒素水平
- <0.01EU/μg purified protein (LAL test, Lonza).
- Top Product
- Discover our top product FliC 蛋白
-
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
-
Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, in vivo on expression of CCL20 or IL-6. TLR5 can be stimulated in vitro by Flagellin (rec.) at concentration range of 5-50ng/ml and in vivo at a starting concentration of 0.1-1μg/mouse. Inflammasome NLRC4 can be activated at a concentration range of 1-5μg/ml on bone-marrow macrophages in the presence of streptolysin O (SLO, 25μg/ml), a protein that allows delivery of exogenous molecules into the cytosol of living cells.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Reconstitute with 100 μL sterile water.
- 浓度
- Lot specific
- 缓冲液
- Lyophilized. Contains PBS.
- 储存条件
- 4 °C,-20 °C
- 储存方法
-
Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C. - 有效期
- 6 months
-
- 抗原
- Flagellin (FliC)
- 别名
- Flagellin (FliC 产品)
- 别名
- hag Protein, flaG Protein, flagellin Protein, flagellin (flic) Protein, flagellin E Protein, flagellin protein flic Protein, fliC Protein, FliC Protein, fliC+F2048 Protein, CJJ81176_1339 Protein, C8J_1256 Protein
- 背景
- Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).
- UniProt
- P06179
- 途径
- Inflammasome
-