WT1 Protein (His tag)
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- 抗原 See all WT1 蛋白
- WT1 (Wilms Tumor 1 (WT1))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This WT1 protein is labelled with His tag.
- 应用范围
- Antibody Production (AbP), Standard (STD)
- 产品特性
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- Recombinant human WT1 / Wilms tumor protein (full length, N-term HIS tag, transcript variant A) protein expressed in E.coli.
- Produced with end-sequenced ORF clone
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product WT1 蛋白
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Wilms Tumor 1 (WT1) (AA 1-449) protein (Strep Tag)
Crystallography grade WT1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Wilms Tumor 1 (WT1) (AA 157-445) protein (His tag)WT1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Wilms Tumor 1 (WT1) (AA 349-439) protein (GST tag)WT1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Wilms Tumor 1 (WT1) (AA 1-302) protein (GST tag)WT1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Wilms Tumor 1 (WT1) (AA 147-446), (partial) protein (His tag)WT1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Wilms Tumor 1 (WT1) (AA 1-449), (full length) protein (His-SUMO Tag)WT1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - 说明
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The tag is located at the N-terminal.
- 限制
- 仅限研究用
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- 浓度
- 50 μg/mL
- 缓冲液
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- WT1 (Wilms Tumor 1 (WT1))
- 别名
- Wt1,wilms Tumor Protein (WT1 产品)
- 别名
- AWT1 Protein, EWS-WT1 Protein, GUD Protein, NPHS4 Protein, WAGR Protein, WIT-2 Protein, WT33 Protein, CWT1 Protein, WT1 Protein, XWT1a Protein, wt-1 Protein, wt1 Protein, xWT1 Protein, WT-1 Protein, gud Protein, wagr Protein, wt33 Protein, xwt1 Protein, wit-2 Protein, si:ch211-160j6.3 Protein, zgc:152697 Protein, D630046I19Rik Protein, Wt-1 Protein, XWT1b Protein, XeWT1 Protein, Wilms tumor 1 Protein, Wilms tumor 1 S homeolog Protein, wilms tumor 1b Protein, Wilms tumor protein Protein, Wilms tumor 1 homolog Protein, Wilms tumor 1 L homeolog Protein, WT1 Protein, wt1.S Protein, wt1 Protein, wt1b Protein, Wt1 Protein, wt1.L Protein
- 背景
- This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.
- 分子量
- 54.1 kDa
- NCBI登录号
- NP_000369
- 途径
- Tube Formation
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