PKC epsilon Protein (DYKDDDDK Tag)
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- 抗原 See all PKC epsilon (PRKCE) 蛋白
- PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- Insect cells (Sf9)
- 标记
- This PKC epsilon protein is labelled with DYKDDDDK Tag.
- 应用范围
- Antibody Production (AbP), Standard (STD)
- 产品特性
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- Recombinant human PRKCE (full length, C-term DDK tag) protein expressed in Sf9 cells.
- Produced with end-sequenced ORF clone
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product PRKCE 蛋白
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Protein Kinase C, epsilon (PRKCE) (AA 1-737) (Active) protein (His-GST)
PRKCE 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
Protein Kinase C, epsilon (PRKCE) (AA 580-737) protein (His tag)PRKCE 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Protein Kinase C, epsilon (PRKCE) (AA 1-737) protein (Strep Tag)Crystallography grade PRKCE 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Protein Kinase C, epsilon (PRKCE) (AA 1-737) protein (Strep Tag)Crystallography grade PRKCE 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - 说明
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The tag is located at the C-terminal.
- 限制
- 仅限研究用
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- 浓度
- 50 μg/mL
- 缓冲液
- 50 mM Tris-HCl, pH 8.0, 100 mM glycine, 10 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))
- 别名
- Prkce (PRKCE 产品)
- 别名
- PKCE Protein, nPKC-epsilon Protein, 5830406C15Rik Protein, PKC[e] Protein, PKCepsilon Protein, Pkce Protein, R75156 Protein, fj59b07 Protein, prkce Protein, wu:fj59b07 Protein, pkce Protein, pkc-epsilon Protein, PRKCH Protein, protein kinase C epsilon Protein, protein kinase C, epsilon Protein, protein kinase C, epsilon a Protein, PRKCE Protein, Prkce Protein, prkcea Protein, prkce Protein
- 背景
- Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior.
- 分子量
- 83.5 kDa
- NCBI登录号
- NP_005391
- 途径
- TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling
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