PPP1R12A Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)
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- 抗原 See all PPP1R12A 蛋白
- PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))
- 蛋白类型
- Recombinant
- 产品特性
- Transcript Variant 2
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This PPP1R12A protein is labelled with Myc-DYKDDDDK Tag.
- 应用范围
- Antibody Production (AbP), Standard (STD)
- 产品特性
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- Recombinant human PPP1R12A / MYPT1 (transcript variant 2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product PPP1R12A 蛋白
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Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (AA 1-1030) protein (Strep Tag)
Crystallography grade PPP1R12A 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (AA 1-1029) protein (Strep Tag)Crystallography grade PPP1R12A 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PPP1R12A 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - 说明
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The tag is located at the C-terminal.
- 限制
- 仅限研究用
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- 浓度
- 50 μg/mL
- 缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))
- 别名
- Ppp1r12a,mypt1 (PPP1R12A 产品)
- 别名
- 1200015F06Rik Protein, 5730577I22Rik Protein, AA792106 Protein, AV099298 Protein, D10Ertd625e Protein, Mypt1 Protein, M130 Protein, MBS Protein, MYPT1 Protein, M110 Protein, MBSP Protein, protein phosphatase 1, regulatory (inhibitor) subunit 12A Protein, protein phosphatase 1 regulatory subunit 12A Protein, protein phosphatase 1, regulatory subunit 12A Protein, Ppp1r12a Protein, PPP1R12A Protein
- 背景
- Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.
- 分子量
- 115.1 kDa
- NCBI登录号
- NP_001137357
- 途径
- M Phase
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