HECW2 Protein (Myc-DYKDDDDK Tag)
-
- 抗原 See all HECW2 蛋白
- HECW2 (HECT, C2 and WW Domain Containing E3 Ubiquitin Protein Ligase 2 (HECW2))
- 蛋白类型
- Recombinant
-
宿主
- 人
-
资源
- HEK-293 Cells
- 标记
- This HECW2 protein is labelled with Myc-DYKDDDDK Tag.
- 应用范围
- Antibody Production (AbP), Standard (STD)
- 产品特性
-
- Recombinant human NEDL2 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product HECW2 蛋白
-
HECT, C2 and WW Domain Containing E3 Ubiquitin Protein Ligase 2 (HECW2) (AA 637-745) protein (GST tag)
HECW2 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
HECT, C2 and WW Domain Containing E3 Ubiquitin Protein Ligase 2 (HECW2) (AA 1-1572) protein (Strep Tag)Crystallography grade HECW2 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
HECT, C2 and WW Domain Containing E3 Ubiquitin Protein Ligase 2 (HECW2) (AA 1-1578) protein (Strep Tag)Crystallography grade HECW2 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
-
- 应用备注
-
Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - 说明
-
The tag is located at the C-terminal.
- 限制
- 仅限研究用
-
- 浓度
- 50 μg/mL
- 缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
-
- 抗原
- HECW2 (HECT, C2 and WW Domain Containing E3 Ubiquitin Protein Ligase 2 (HECW2))
- 别名
- Nedl2 (HECW2 产品)
- 别名
- HECW2 Protein, NEDL2 Protein, A730039N16Rik Protein, D030049F17Rik Protein, Gm971 Protein, Nedl2 Protein, mKIAA1301 Protein, HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 Protein, E3 ubiquitin-protein ligase HECW2 Protein, HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2b Protein, Hecw2 Protein, HECW2 Protein, LOC531691 Protein, hecw2b Protein, hecw2 Protein
- 背景
- This gene encodes a member of a family of E3 ubiquitin ligases which plays an important role in the proliferation, migration and differentiation of neural crest cells as a regulator of glial cell line-derived neurotrophic factor (GDNF)/Ret signaling. This gene also plays an important role in angiogenesis through stabilization of endothelial cell-to-cell junctions as a regulator of angiomotin-like 1 stability. The encoded protein contains an N-terminal calcium/lipid-binding (C2) domain involved in membrane targeting, two-four WW domains responsible for cellular localization and substrate recognition, and a C-terminal homologous with E6-associated protein C-terminus (HECT) catalytic domain. Naturally occurring mutations in this gene are associated with neurodevelopmental delay, hypotonia, and epilepsy. The decreased expression of this gene in the aganglionic colon is associated with Hirschsprung&aposs disease. Alternative splicing results in multiple transcript variants.
- 分子量
- 175.6 kDa
- NCBI登录号
- NP_065811
-
