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M-CSF/CSF1 Protein (Transcript Variant 4)

This Recombinant M-CSF/CSF1 protein is expressed in HEK-293 Cells.
产品编号 ABIN2725102
发货至: 中国

Quick Overview for M-CSF/CSF1 Protein (Transcript Variant 4) (ABIN2725102)

抗原

See all M-CSF/CSF1 (CSF1) 蛋白
M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

蛋白类型

Recombinant

生物活性

Active

宿主

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资源

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HEK-293 Cells

应用范围

Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)

纯度

> 98 % , as determined by Coomassie stained SDS-PAGE.
  • 产品特性

    Transcript Variant 4

    特异性

    Optimal preservation of protein structure, post-translational modifications and functions.

    产品特性

    • Recombinant human M-CSF / CSF-1 (transcript variant 4) protein expressed in HEK293.
    • Produced with end-sequenced ORF clone
    • Tested for bioactivity.

    内毒素水平

    Less than 0.01 ng per μg protein as determined by the LAL method.

    Biological Activity Comment

    The ED50 is 0.5 - 2 ng/ml, corresponding to a specific activity of 0.5 - 2 x 10^6 units/mg, determined by M-NFS60 cell proliferation induced by human M-CSF in a dose dependent manner.
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  • 应用备注

    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Protein-protein interaction
    In vitro biochemical assays and cell-based functional assays

    限制

    仅限研究用
  • 浓度

    > 50 μg/mL

    缓冲液

    1 x PBS

    储存条件

    -80 °C

    储存方法

    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • 抗原

    M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

    别名

    M-Csf,csf-1

    背景

    The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants.

    分子量

    20.6 kDa

    NCBI登录号

    NP_757351

    途径

    RTK signaling
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