HSP70 1A Protein (Myc-DYKDDDDK Tag)
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- 抗原 See all HSP70 1A (HSPA1A) 蛋白
- HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This HSP70 1A protein is labelled with Myc-DYKDDDDK Tag.
- 应用范围
- Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
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- Recombinant human Heat shock 70 kDa protein 1A (HSPA1A) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Biological Activity Comment
- Higher specific activity than E. coli derived HSP70: human recombinant Hsp70 was compared side-by-side with E. coli derived Hsp70 in a firefly luciferase refolding assay. Percentage of refolding is relative to an identical load of non-denatured luciferase in the reaction. The human cell produced Hsp70 is approximately 30% more active than the bacterial produced Hsp70
- Top Product
- Discover our top product HSPA1A 蛋白
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Heat Shock 70kDa Protein 1A (HSPA1A) (AA 291-641) protein (His-IF2DI Tag)
HSPA1A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Heat Shock 70kDa Protein 1A (HSPA1A) (Active) protein (His tag)HSPA1A 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 85 % as determined by reducing SDS-PAGE. Active
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 说明
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The tag is located at the C-terminal.
- 限制
- 仅限研究用
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- 浓度
- > 50 μg/mL
- 缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))
- Abstract
- HSPA1A 产品
- 别名
- hsp70-4 Protein, hspa1a Protein, Hsp70-3 Protein, Hsp70.3 Protein, Hsp72 Protein, hsp68 Protein, hsp70A1 Protein, HSP72 Protein, Hsp70-1 Protein, Hspa1 Protein, Hspa1b Protein, hsp70-1 Protein, hsp70-1a Protein, hsp70i Protein, hsp72 Protein, hspa1 Protein, hspa1b Protein, HSP70-1 Protein, HSP70-1A Protein, HSP70I Protein, HSPA1 Protein, HSPA1A Protein, HSPA1B Protein, HSP70-2 Protein, HSPA2 Protein, HSP70 Protein, hspA1A Protein, heat shock cognate 70-kd protein, tandem duplicate 3 Protein, heat shock 70 kDa protein 1 Protein, heat shock 70 kDa protein II Protein, heat shock protein 1A Protein, heat shock 70kD protein 1A Protein, heat shock protein family A (Hsp70) member 1A S homeolog Protein, heat shock protein family A (Hsp70) member 1A Protein, heat shock protein 70.2 Protein, heat shock 70kDa protein 1A Protein, heat shock 70 kDa protein 1B Protein, heat shock protein 70.1 Protein, hsp70.3 Protein, LOC100023597 Protein, LOC100554002 Protein, LOC100608888 Protein, Hspa1a Protein, hspa1a.S Protein, HSPA1A Protein, HSP70.2 Protein, LOC100354037 Protein, HSP70.1 Protein
- 背景
- This intronless gene encodes a 70 kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
- 分子量
- 69.9 kDa
- NCBI登录号
- NP_005336
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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