ADD3 Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag)
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- 抗原 See all ADD3 蛋白
- ADD3 (Adducin 3 (Gamma) (ADD3))
- 蛋白类型
- Recombinant
- 产品特性
- Transcript Variant 3
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This ADD3 protein is labelled with Myc-DYKDDDDK Tag.
- 应用范围
- Antibody Production (AbP), Standard (STD)
- 产品特性
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- Recombinant human Gamma-adducin (ADD3) (transcript variant 3) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product ADD3 蛋白
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Adducin 3 (Gamma) (ADD3) (AA 462-560) protein (GST tag)
ADD3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Adducin 3 (Gamma) (ADD3) (AA 2-706) protein (His tag)Crystallography grade ADD3 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Adducin 3 (Gamma) (ADD3) (AA 2-706) protein (His tag)Crystallography grade ADD3 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Adducin 3 (Gamma) (ADD3) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)ADD3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - 说明
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The tag is located at the C-terminal.
- 限制
- 仅限研究用
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- 浓度
- 50 μg/mL
- 缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- ADD3 (Adducin 3 (Gamma) (ADD3))
- 别名
- gamma-Adducin (Add3) (ADD3 产品)
- 别名
- gamma-adducin Protein, add3 Protein, MGC89418 Protein, ADD3 Protein, DKFZp459E0630 Protein, ADDL Protein, AI463285 Protein, R75380 Protein, adducin 3 (gamma) Protein, adducin 3 Protein, adducin 3 L homeolog Protein, ADD3 Protein, add3 Protein, add3.L Protein, Add3 Protein
- 背景
- Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known.
- 分子量
- 75.5 kDa
- NCBI登录号
- NP_001112
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