EBI3 Protein (His tag)
(1 reference)-
- 抗原 See all EBI3 (IL-27b) 蛋白
- EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- CHO Cells
- 标记
- This EBI3 protein is labelled with His tag.
- 应用范围
- Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
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- Recombinant human EBI3 / IL27B protein expressed in CHO cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 95 % , as determined by Coomassie stained SDS-PAGE.
- 内毒素水平
- Less than 0.01 ng per μg protein as determined by the LAL method.
- Biological Activity Comment
- The ED50 is 60 - 90 ng/ml, corresponding to a specific activity of 1.1 - 1.7 x 10^4 units/mg, determined by mouse splenocytes IL-2 inhibition induced by hIL-27 in mouse splenocytes activated with anti-CD3 and anti-CD28 antibodies.
- Top Product
- Discover our top product IL-27b 蛋白
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Interleukin-27 subunit beta (IL-27b) (AA 1-229) protein (GST tag)
IL-27b 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Interleukin-27 subunit beta (IL-27b) (AA 23-228) protein (His-IF2DI Tag)IL-27b 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin-27 subunit beta (IL-27b) (AA 129-229) protein (GST tag)IL-27b 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 说明
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The tag is located at the C-terminal.
- 限制
- 仅限研究用
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- 浓度
- > 50 μg/mL
- 缓冲液
- 20 mM NaHPO4, pH 6, 0.60 M NaCl.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "A novel IL-23p19/Ebi3 (IL-39) cytokine mediates inflammation in Lupus-like mice." in: European journal of immunology, Vol. 46, Issue 6, pp. 1343-50, (2016) (PubMed).
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: "A novel IL-23p19/Ebi3 (IL-39) cytokine mediates inflammation in Lupus-like mice." in: European journal of immunology, Vol. 46, Issue 6, pp. 1343-50, (2016) (PubMed).
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- 抗原
- EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))
- 别名
- Ebi3,il27b (IL-27b 产品)
- 别名
- IL-27B Protein, IL27B Protein, EBI3 Protein, il-27rb Protein, si:dkey-97a13.4 Protein, EBI-3 Protein, IL-27 Protein, Epstein-Barr virus induced 3 Protein, Epstein-Barr virus induced gene 3 Protein, EBI3 Protein, ebi3 Protein, Ebi3 Protein
- 物质类
- Viral Protein
- 背景
- This gene was identified by its induced expression in B lymphocytes in response Epstein-Barr virus infection. It encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form interleukin 27 (IL-27). IL-27 regulates T cell and inflammatory responses, in part by activating the Jak/STAT pathway of CD4+ T cells.
- 分子量
- 50 kDa
- NCBI登录号
- NP_005746
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