LAP3 Protein (Myc-DYKDDDDK Tag)
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- 抗原 See all LAP3 蛋白
- LAP3 (Cytosol Aminopeptidase (LAP3))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This LAP3 protein is labelled with Myc-DYKDDDDK Tag.
- 应用范围
- Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
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- Recombinant human Cytosol aminopeptidase protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Biological Activity Comment
- LAP3 activity verified in a biochemical assay: Leucine aminopeptidase 3 (LAP3, ) activity was measured in a fluorescent biochemical assay. LAP3 catalyzes the removal of unsubstituted N-terminal amino acids from various peptides and is most active on leucine. LAP3 activity was measured in a 100 μl reaction mixture containing 1 mM L-leucine 7-amido-4-methyl coumarin (Leu-AMC), 50 mM Tris, pH 8.0, 4 mM MgCl2, and 1 mM MnCl2. Cleavage of leucine from the AMC moiety results in a strong increase in fluorescence intensity. Fluorescence was measured over time with an excitation wavelength of 380 nm and an emission wavelength of 460 nm. The activity of the enzyme in this system remained constant over six hours.
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- Discover our top product LAP3 蛋白
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Cytosol Aminopeptidase (LAP3) (AA 252-368) protein (His-IF2DI Tag)
LAP3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Cytosol Aminopeptidase (LAP3) (AA 1-519) protein (GST tag)LAP3 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Cytosol Aminopeptidase (LAP3) (AA 420-519) protein (GST tag)LAP3 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Cytosol Aminopeptidase (LAP3) (AA 1-519) protein (His tag)LAP3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Cytosol Aminopeptidase (LAP3) (AA 1-519) protein (Strep Tag)Crystallography grade LAP3 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Cytosol Aminopeptidase (LAP3) (AA 1-519) protein (Strep Tag)Crystallography grade LAP3 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Cytosol Aminopeptidase (LAP3) (AA 25-64), (partial) protein (His-SUMO Tag)LAP3 宿主: Cow 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 说明
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The tag is located at the C-terminal.
- 限制
- 仅限研究用
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- 浓度
- > 50 μg/mL
- 缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- LAP3 (Cytosol Aminopeptidase (LAP3))
- 别名
- Cytosol Aminopeptidase (LAP3 产品)
- 别名
- LAP Protein, LAPEP Protein, PEPS Protein, 2410015L10Rik Protein, AA410100 Protein, LAP-3 Protein, Lap Protein, Lapep Protein, Pep-7 Protein, Pep-S Protein, Pep7 Protein, Peps Protein, cytosol aminopeptidase Protein, leucine aminopeptidase 3 Protein, pepA Protein, Plabr_1567 Protein, Dester_0800 Protein, Weevi_0186 Protein, Marky_0570 Protein, Halhy_2623 Protein, FsymDg_3217 Protein, Flexsi_2186 Protein, Runsl_3980 Protein, lap3 Protein, LAP3 Protein, Lap3 Protein
- 背景
- Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. [UniProtKB/Swiss-Prot Function]
- 分子量
- 56 kDa
- NCBI登录号
- NP_056991
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