IL12 Protein (C-Term, Extracellular Domain)
Quick Overview for IL12 Protein (C-Term, Extracellular Domain) (ABIN1684636)
抗原
See all IL12 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- C-Term, Extracellular Domain
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特异性
- DNA sequence encoding extracellular domain of Human IL-12B fused to a C-terminal polyHis tag was expressed in HEK cells.
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产品特性
- Recombinant Human Interleukin-12B p40 subunit is a monomer consisting of 306 amino acid residue and migrates as an approximately 46 kDa protein under reducing conditions in SDS-PAGE.
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过滤
- 0.2 μm filtered
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内毒素水平
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
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Interleukin 12 (IL12) (AA 23-328) protein (His-Avi Tag)
IL12 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
Interleukin 12 (IL12) (AA 23-328) protein (His-Avi Tag,Biotin)IL12 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 12 (IL12) (AA 23-219), (AA 23-328) (Active) proteinIL12 宿主: 人 宿主: HEK-293T Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 12 (IL12) (AA 23-215) protein (His tag)IL12 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 12 (IL12) (AA 23-328) (Active) protein (His tag,DYKDDDDK Tag)IL12 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 12 (IL12) (AA 23-328) (Active) protein (His tag,AVI tag,DYKDDDDK Tag,Biotin)IL12 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 12 (IL12) proteinIL12 宿主: 小鼠 宿主: 人细胞 Recombinant > 90 % as determined by SDS-PAGE
Interleukin 12 (IL12) protein (His tag)IL12 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Interleukin 12 (IL12) (AA 23-219) protein (Fc-His Tag)IL12 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 12 (IL12) (Active) protein (His tag)IL12 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90% by SDS-PAGE SDS, AcA Active
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说明
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The activity was determined by its ability to bind recombinant Human IL-12RB1 in functional ELISA. Protein Sequence 10 0 30 40 50 60 MCHQQLVISW FSLVFLASPL VAIWELKKDV YVVELDWYPD APGEMVVLTC DTPEEDGITW 70 80 90 100 110 120 TLDQSSEVLG SGKTLTIQVK EFGDAGQYTC HKGGEVLSHS LLLLHKKEDG IWSTDILKDQ 130 140 150 160 170 180 KEPKNKTFLR CEAKNYSGRF TCWWLTTIST DLTFSVKSSR GSSDPQGVTC GAATLSAERV 190 00 10 20 30 40 RGDNKEYEYS VECQEDSACP AAEESLPIEV MVDAVHKLKY ENYTSSFFIR DIIKPDPPKN 50 60 70 80 90 300 LQLKPLKNSR QVEVSWEYPD TWSTPHSYFS LTFCVQVQGK SKREKKDRVF TDKTSATVIC 310 320 330 RKNASISVRA QDRYYSSSWS EWASVPCS. Complete precursor sequence shown, expressed chain highlighted
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
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缓冲液
- PBS solution pH 7.4.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- The lyophilized antibody is stable for at least 1 year from date of receipt at -20 °C. Upon reconstitution, this antibody can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months without detectable loss of activity.
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有效期
- 12 months
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- IL12 (Interleukin 12 (IL12))
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别名
- IL-12
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背景
- IL-6 is produced by many different cell types. The main sources in vivo are stimulated monocytes, fibroblasts, and endothelial cells. Macrophages, T-cells and B-lymphocytes, granulocytes, smooth muscle cells, eosinophils, chondrocytes, osteoblasts, mast cells, glial cells, and keratinocytes also produce IL6 after stimulation. IL6 is a member of a family of cytokines, which also includes LIF, CNTF, Oncostatin M, IL11, and CT-1. All known members of the IL6 cytokine family induce hepatic expression of acute phase proteins. The IL6 receptor is expressed on T-cells, mitogen-activated B-cells, peripheral monocytes and some macrophage- and B-cell derived tumor cell types. It is not expressed in resting B-cells but in resting T-cells. The IL6 receptor is a strongly glycosylated protein of 80 kDa and a length of 449 amino acids. It has been designated CD126.
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UniProt
- P29460
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途径
- JAK/STAT Signaling, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
抗原
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