DOT1L Protein (AA 1-416) (GST tag)
DOT1L
宿主: 人
宿主: 大肠杆菌（E. Coli）
Recombinant
EAA, ScA
Active
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- 抗原 See all DOT1L 蛋白
- DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-416
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This DOT1L protein is labelled with GST tag.
- 应用范围
- Enzyme Activity Assay (EAA), Screening Assay (ScA)
- 产品特性
- Recombinant DOT1L (1-416), an active truncation that includes amino acids 1-416 (accession number NP_115871.1) ,was expressed in E. coli cells and contains an N-terminal GST tag with an observed molecular weight of 72 kDa.
- Top Product
- Discover our top product DOT1L 蛋白
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DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L) (AA 3-108) protein (GST tag)
DOT1L 宿主: 人 宿主: 小麦胚 Recombinant ELISA, AA, AP, WB
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- 应用备注
- Recombinant DOT1L (1-416), active protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K79 methyltransferase HMT Assay Conditions: 50 mM TrisCl, pH 8.6, 0.02 % Triton X-100, 2 mM MgCl2, 1 mM TCEP, 100 μM SAM, 30 ng/μl Recombinant Nucleosomes, 30 ng/μl DOT1L (1-416 aa) at 2 hours at room temperature. Activity was detected by fluorography.
- 限制
- 仅限研究用
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- 注意事项
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- 储存条件
- -80 °C
- 储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- 抗原
- DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))
- 别名
- DOT1L (DOT1L 产品)
- 别名
- NV11307 Protein, DOT1L Protein, DOT1 Protein, KMT4 Protein, A630076O07 Protein, AW907654 Protein, Dot1 Protein, mDot1 Protein, histone-lysine N-methyltransferase, H3 lysine-79 specific Protein, DOT1 like histone lysine methyltransferase Protein, histone methyltransferase Protein, DOT1-like, histone H3 methyltransferase (S. cerevisiae) Protein, LOC100116648 Protein, DOT1L Protein, DOT1 Protein, Dot1l Protein
- 背景
- DOT1L (Disruptor of Telomeric silencing 1-like, also known as KMT4) is the mammalian homologue of the yeast Dot1 protein and the Drosophila Grappa protein. DOTL1 is the enzyme responsible for lysine 79 methylation of histone H3. DOT1L can promote an oncogenic pattern of gene expression through binding with several MLL fusion partners found in acute leukemia, thus leading to aberrant methylation of lysine 79 at MLL target genes. In contrast to most other histone lysine methyltransferases, DOT1L does not contain a SET domain. Biochemical studies suggest that recombinant DOT1L is active on recombinant nucleosomes.
- 分子量
- 72 kDa
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