EZH2 Protein (Lys27) (DYKDDDDK Tag,His tag)
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- 抗原 See all EZH2 蛋白
- EZH2 (Enhancer of Zeste Homolog 2 (EZH2))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- Lys27
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宿主
- 人
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资源
- Baculovirus
- 标记
- This EZH2 protein is labelled with DYKDDDDK Tag,His tag.
- 应用范围
- Enzyme Activity Assay (EAA)
- 产品特性
- The EZH2 protein complex, active contains EZH2, EED and Suz12 which are the minimal components required for histone methyltransferase activity. EZH2 is specific for H3 lysine 27, a mark of repressed regions of chromatin. Recombinant human EZH2 (accession number NM_004456, amino acids 2-end with an N-terminal His tag, molecular weight 86 kDa) was coexpressed in a baculovirus expression system along with human EED (accession number NM_003797, amino acids 2-end with an N-terminal FLAG tag, molecular weight 51 kDa) and human Suz12 (accession number NM_015355, amino acids 2-end with an N-terminal His tag, molecular weight 87 kDa).
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- Discover our top product EZH2 蛋白
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Enhancer of Zeste Homolog 2 (EZH2) (AA 1-751) protein (GST tag)
EZH2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Enhancer of Zeste Homolog 2 (EZH2) (AA 1-110) protein (GST tag)EZH2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Enhancer of Zeste Homolog 2 (EZH2) (AA 1-746) protein (Strep Tag)Crystallography grade EZH2 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Enhancer of Zeste Homolog 2 (EZH2) (AA 1-746) protein (Strep Tag)Crystallography grade EZH2 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Enhancer of Zeste Homolog 2 (EZH2) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)EZH2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Recombinant EZH2 protein complex is suitable for use in histone methyltransferase assays. This protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Specific Activity: 0.08 pmol/min/μg. Assay conditions: Prepare a 50 μL reaction mix (20 mM phosphate pH 7.4, 0.05 % Tween-20, 40 μM S-adenosylmethionine, and 250-2000 ng enzyme complex) and add to substrate-coated wells with the substrate on a Thermo Neutravidin black plate. Incubate for 1 hr. Add anti-methyl histone H3K27 antibody and incubate 1hr. Next, incubate 1h with the HRP-labeled secondary antibody followed by chemiluminescent detection.
- 限制
- 仅限研究用
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- 注意事项
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- 储存条件
- -80 °C
- 储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- 抗原
- EZH2 (Enhancer of Zeste Homolog 2 (EZH2))
- 别名
- EZH2 (EZH2 产品)
- 别名
- ENX-1 Protein, ENX1 Protein, EZH1 Protein, EZH2b Protein, KMT6 Protein, KMT6A Protein, WVS Protein, WVS2 Protein, eya2 Protein, im:7149338 Protein, zgc:136376 Protein, zgc:152758 Protein, EZH2 Protein, enx-1 Protein, enx1 Protein, ezh1 Protein, kmt6 Protein, xez Protein, Enx-1 Protein, Enx1h Protein, mKIAA4065 Protein, ezh2 Protein, olezh2 Protein, enhancer of zeste 2 polycomb repressive complex 2 subunit Protein, enhancer of zeste 2 polycomb repressive complex 2 subunit L homeolog Protein, enhancer of zeste 2 polycomb repressive complex 2 subunit S homeolog Protein, EZH2 Protein, ezh2 Protein, Ezh2 Protein, ezh2.L Protein, ezh2.S Protein
- 背景
- EZH2, EED and Suz12 are present in the PRC protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2 - (Enhancer of Zeste homolog 2, also designated Enx1 and SETDB1) is a human homolog of the Drosophila Enhancer of Zeste protein. It contains a SET domain that catalyzes the mono-, di- and trimethylation of Histone H3 Lys27. Polycomb group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. Deregulation of EZH2 is linked to a variety of cancers.
- 途径
- Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation
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