HIV-1 Tat 蛋白
宿主: Human Immunodeficiency Virus (HIV)
宿主: 大肠杆菌（E. Coli）
Recombinant
> 90 %
WB, SDS
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- 抗原 See all HIV-1 Tat 蛋白
- HIV-1 Tat
- 蛋白类型
- Recombinant
- 宿主
- Human Immunodeficiency Virus (HIV)
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS)
- 纯度
- > 90 %
- Top Product
- Discover our top product HIV-1 Tat 蛋白
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- The protein should be reconstituted in apirogenic sterile water or 1X PBS.
- 缓冲液
- 0.1 % glycerol
- 储存条件
- 4 °C
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- 抗原
- HIV-1 Tat
- 别名
- HIV1 Tat (HIV-1 Tat 产品)
- 物质类
- Viral Protein
- 背景
- The redox state of cysteine-rich region of HIV tat protein is known to play a crucial role in tat biological activity. The cysteine-rich region, encompassing seven cysteine residues, is highly conserved among the different HIV-1 strains and isolates. Its redox state is known to be important for protein function and activity. In particular, variation of the tat cysteine motif known as "cysteine 30-cysteine 31" (C30C31) is associated with clade-specific tat neurotoxicity. Formation of tat homodimers also depends on the oxidation state of the protein, as dimerization requires metal ions binding to free sulphydryl groups present in the cysteine region. In the presence of oxygen and in absence of reducing agents, tat protein rapidly oxidises forming intra-molecular disulfide bonds which prevent the interaction with metal ions. Further, oxidation induces the formation of inter-molecular disulfide bonds causing tat multimerization and aggregation. Due to these effects, oxidation hampers or abrogates tat biological activity.
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