IL-15 Protein (AA 49-162)
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- 抗原 See all IL-15 (IL15) 蛋白
- IL-15 (IL15) (Interleukin 15 (IL15))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 49-162
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Flow Cytometry (FACS)
- 纯度
- >98 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- 内毒素水平
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Less than 0.1 ng per μg of protein.
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- Discover our top product IL15 蛋白
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Interleukin 15 (IL15) protein
IL15 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by SEC-HPLC
Interleukin 15 (IL15) (AA 50-162) (Active) protein (His tag)IL15 宿主: 人 宿主: 大肠杆菌（E. Coli） > 97 % AcA, CC Active
Interleukin 15 (IL15) (AA 49-162) protein (His-IF2DI Tag)IL15 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 15 (IL15) (AA 49-162) protein (His-IF2DI Tag)IL15 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 15 (IL15) (AA 1-162) protein (GST tag)IL15 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 15 (IL15) (AA 30-129) protein (GST tag)IL15 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 15 (IL15) (AA 49-162) (Active) protein (His tag)IL15 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Interleukin 15 (IL15) proteinIL15 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant
Interleukin 15 (IL15) (AA 49-162) (Active) protein (Fc Tag)IL15 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162) (Active) protein (His tag)IL15 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: ED50 is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2.0 x 106 units/mg as determined by the dose-dependent stimulation of the proliferation of mouse CTLL-2 cells.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.
- 缓冲液
- Lyophilized, carrier-free.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored at -20°C or -70°C.
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- 抗原
- IL-15 (IL15) (Interleukin 15 (IL15))
- 别名
- IL-15 (IL15 产品)
- 别名
- IL15 Protein, AI503618 Protein, IL-15 Protein, interleukin 15 Protein, IL15 Protein, Il15 Protein
- 背景
- IL-15 was discovered in the supernatant from a simian kidney epithelial cell line CV-1/EBNA, as a soluble factor capable of supporting proliferation of the IL-2-dependent cell line, CTLL-2. IL-15 is a regulatory cytokine, and it is produced by dendritic cells, epithelial cells, human stromal cell line (IMTLH), fibroblasts, and monocytes. IL-15 plays an important role in immune response and shares many functions with IL-2, for example, stimulating the proliferation of activated T cells, NK cells and B cells, and inducing immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand. In addition, IL-15 promotes the development of dendritic cells, activates human neutrophils and induces the production of proinflammatory cytokines from macrophages. IL-15 acts as a bridge between innate and adaptive immunity because of its diverse roles in the immune system. IL-15 binds to heterotrimeric receptors composed of IL-15Rα, IL-15Rβ, and IL-15Rγc. IL-15 shares with IL-2 the receptor chains β and γc. IL-15 is normally not secreted in soluble form but is held on the cell surface bound to a unique receptor, IL-15Rα, especially on dendritic cells. Cell-bound IL-15 then is presented in trans to T cells and NK cells and is recognized by the γc receptor on these cells, such recognition maintains cell survival and intermittent proliferation.
- 分子量
- The 115 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 12.9 kDa. The predicted N-terminal amino acid is Met.
- 途径
- JAK/STAT Signaling, Glycosaminoglycan Metabolic Process
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