Podoplanin Protein (PDPN) (AA 12-137, C-Term) (Fc Tag)
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- 抗原 See all Podoplanin (PDPN) 蛋白
- Podoplanin (PDPN)
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 12-137, C-Term
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This Podoplanin protein is labelled with Fc Tag.
- 应用范围
- Flow Cytometry (FACS)
- 纯度
- > 95 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
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Less than 0.1 EU per μg of protein as determine by the LAL method.
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Podoplanin (PDPN) protein (Fc Tag)
PDPN 宿主: 小鼠 宿主: 人细胞 Recombinant > 90 % as determined by reducing SDS-PAGE.
Podoplanin (PDPN) (AA 99-207) protein (His tag)PDPN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Podoplanin (PDPN) (AA 23-141) protein (His tag)PDPN 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Podoplanin (PDPN) protein (Fc Tag,ECD)PDPN 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
Podoplanin (PDPN) (AA 1-238) protein (GST tag)PDPN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Podoplanin (PDPN) protein (His tag)PDPN 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 92 % as determined by SDS-PAGE
Podoplanin (PDPN) (Active) protein (His tag,Fc Tag)PDPN 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
Podoplanin (PDPN) (AA 23-131) protein (His tag)Crystallography grade PDPN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Podoplanin (PDPN) (AA 23-162) protein (rho-1D4 tag)Crystallography grade PDPN 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Podoplanin (PDPN) (AA 23-172) protein (rho-1D4 tag)Crystallography grade PDPN 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: Recombinant mouse podoplanin, when coated on a plate (2 μg/ml, 100 μl/well), is able to bind mouse CLEC-2 in a dose dependent manner with an apparent kD <1.0 nM.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
- 浓度
- 200 μg/mL
- 缓冲液
- 0.22 μm filtered protein solution is in PBS.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- 抗原
- Podoplanin (PDPN)
- 别名
- Podoplanin (PDPN 产品)
- 别名
- PDPN Protein, AGGRUS Protein, GP36 Protein, GP40 Protein, Gp38 Protein, HT1A-1 Protein, OTS8 Protein, PA2.26 Protein, T1A Protein, T1A-2 Protein, OTS-8 Protein, RANDAM-2 Protein, T1-alpha Protein, T1a Protein, T1alpha Protein, E11 Protein, RTI40 Protein, podoplanin Protein, PDPN Protein, Pdpn Protein
- 背景
- Podoplanin, also termed GP38, is a 38 kD type I transmembrane sialoglycoprotein and a member of the podoplanin family. Mouse and human GP38 share 46 % amino acid sequence homology. Alternative splicing transcripts, encoding different isoforms of GP38, have been identified. Structurally, GP38 has a 119 amino acid extracellular domain (ECD), a 21 amino acid transmembrane region, and a 10 amino acid cytoplasmic tail. This type I protein is heavily O-glycosylated and has a diverse distribution in human tissues, including glomerular epithelial cells, lung alveolar cells, lymphatic endothelial cells, and neural tissues. GP38 is also expressed by tumor cells such as testicular seminomas, squamous cell carcinomas, colorectal tumors, and brain tumors. Therefore, this protein is a target of antibody therapy in cancer treatment. GP38 induces platelet aggregation and tumor metastasis through its association with CLEC2. The binding of GP38 and CLEC2 is important for maintaining high endothelial cell venule integrity. Also, GP38 is necessary for lymphatic vessel formation, normal lung cell proliferation, and alveolus formation at birth. In addition, GP38 was shown to be a novel marker for immature hepatic progenitor cells in fetal livers. GP38 is upregulated in Th17 cells in the arthritic joints in SKG mice, suggesting that this protein could be a useful Th17 cell marker for diagnosing pathological conditions of autoimmune diseases, including rheumatoid arthritis.
- 分子量
- This 362 amino acid recombinant protein predicted has a molecular mass of approximately 39.8 kDa. The protein migrates at about 60 kDa by SDS-PAGE in DTT-reducing conditions and about 130 kDa in non-reducing conditions. The predicted N-terminal amino acid
- 途径
- Dicarboxylic Acid Transport
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