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M-CSF/CSF1 Protein (AA 33-262)

Name: M-CSF/CSF1 Protein (AA 33-262)
SKU: ABIN2666672
Availability: OutOfStock

This Recombinant M-CSF/CSF1 protein is expressed in HEK-293 Cells.

产品编号 ABIN2666672

发货至: 中国

Quick Overview for M-CSF/CSF1 Protein (AA 33-262) (ABIN2666672)

抗原

See all M-CSF/CSF1 (CSF1) 蛋白

M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

蛋白类型

Recombinant

生物活性

Active

宿主

小鼠

资源

HEK-293 Cells

应用范围

Flow Cytometry (FACS)

纯度

> 98 % , as determined by Coomassie stained SDS-PAGE.

Discover our top product CSF1 蛋白

产品特性

AA 33-262

过滤

0.22 μm filtered

内毒素水平

Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-190) protein
CSF1 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-190) protein (His-Avi Tag)
CSF1 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-255) protein (Fc Tag)
CSF1 宿主: 人宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-255) protein (His tag)
CSF1 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE

Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-255) protein (His tag)
CSF1 宿主: 人宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-190) protein (His-Avi Tag,Biotin)
CSF1 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-190), (Isoform 3) (Active) protein
CSF1 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as analyzed by non-reducing SDS-PAGE. Active

Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-187) (Active) protein
CSF1 宿主: 小鼠宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active

Colony Stimulating Factor 1 (Macrophage) (CSF1) (Active) protein
CSF1 宿主: 小鼠宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE Active

Colony Stimulating Factor 1 (Macrophage) (CSF1) (AA 33-255) protein (mFc Tag)
CSF1 宿主: 人宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

应用备注

Optimal working dilution should be determined by the investigator.

说明

Biological activity: ED50 =2 - 6 ng/ml, corresponding to a specific activity of 1.6 - 5 x 105 units/mg, as determined by M-NFS60 cell proliferation induced by mouse M-CSF in a dose dependent manner.

限制

仅限研究用
状态

Liquid

溶解方式

For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

缓冲液

0.22 μm filtered protein solution is in PBS.

注意事项

Avoid repeated freeze/thaw cycles.

储存条件

-20 °C

储存方法

Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
抗原

M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

别名

M-CSF

背景

M-CSF was first characterized as a glycoprotein that induces monocyte and macrophage colony formation from precursors in murine bone marrow cultures. M-CSF binds CD14+ monocytes and promotes the survival/proliferation of peripheral blood monocytes. In addition, M-CSF enhances inducible monocyte functions including phagocytic activity, microbial killing, and cytotoxicity for tumor cells as well as induces the synthesis of inflammatory cytokines such as IL-1, TNFα, and IFNγ in monocytes. Multiple CSF1 mRNA species have been described that arise from alternative splicing in exon 6 and the alternative use of the 3' end of exons 9 or 10. As a result, two distinct CSF1 protein products are encoded by these transcripts: a cell-surface or membrane-bound form of CSF1 (mCSF1) and a soluble form (sCSF1). Uterine sCSF1 is highly increased during pregnancy. On the contrary, uterine mCSF1 remains low during pregnancy. High levels of M-CSF have been associated to different pathologies such as pulmonary fibrosis and atherosclerosis. M-CSF binds to its receptor M-CSFR, and this receptor is shared by a second ligand, IL-34. Mouse M-CSF and IL-34 exhibit cross-species specificity, both bind to the human and mouse M-CSF receptors. IL-34 can regulate myeloid development and substitute for CSF-1 in vivo. IL-34 has overlapping but not identical biological activities as M-CSF.

分子量

The 251 amino acid recombinant protein has a predicted molecular mass of approximately 28.2 kDa. The DTT-reduced and non-reduced protein migrate at approximately 50 kDa and 100 kDa respectively by SDS-PAGE. The N-terminal contains a His9-(SGGG)2-IEGR-tag.

途径

RTK signaling