IL-3 Protein (AA 20-152, C-Term)
Quick Overview for IL-3 Protein (AA 20-152, C-Term) (ABIN2666658)
抗原
See all IL-3 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 20-152, C-Term
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Interleukin 3 (IL-3) (AA 20-152) protein (His-Avi Tag)
IL-3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 20-143) protein (His tag)IL-3 宿主: Cynomolgus 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 20-152) protein (His-Avi Tag,Biotin)IL-3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) protein (His tag)IL-3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Interleukin 3 (IL-3) (AA 27-166) protein (Fc Tag)IL-3 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 33-166) (Active) proteinIL-3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE analysis. Active
Interleukin 3 (IL-3) (AA 27-166) protein (His-IF2DI Tag)IL-3 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interleukin 3 (IL-3) (AA 33-166) (Active) protein (His tag)IL-3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Interleukin 3 (IL-3) protein (His tag)IL-3 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Interleukin 3 (IL-3) (AA 1-152) protein (GST tag)IL-3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: ED50 = 0.10 - 0.30 ng/ml, corresponding to a specific activity of 0.33 - 1.0 x 107 units/mg, as determined by the dose dependent stimulation of TF-1 cell proliferation assay. Under this assay the bioactivity is equivalent to competit
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
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缓冲液
- 0.22 μm filtered protein solution is in 20 mM NaH2PO4, 0.30 M NaCl, pH 6.0.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- IL-3 (Interleukin 3 (IL-3))
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别名
- IL-3
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背景
- Interleukin-3 is a hemopoietic growth factor involved in the survival, proliferation, and differentiation of multipotent hemopoietic cells. IL-3 is the most potent growth factor for basophils, followed by granulocyte-macrophage colony-stimulating factor and IL-5. These cytokines also act on mature basophils through specific receptors, thereby mediating adhesion, migration, and releasability. IL-3 is highly expressed by mast cells, and large and rapidly released amounts of autocrine IL-3 production is responsible for mast cell survival by IgE in the absence of antigen. IL-3 has also been implicated in the pathogenesis of several chronic inflammatory diseases, including asthma, atherosclerosis, and neurodegenerative disorders, such as multiple sclerosis. IL-3 stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. IL-3 plays a vital role in stimulating basophils and mast cell responses to parasite infections, and since basophils play an important role in Th2 immune responses, IL-3 is a critical regulator of allergic inflammation. IL-3 is expressed in the major embryonic vessels and regulates the survival and proliferation of hematopoietic stem cells in the early stages of embryonic development.
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分子量
- The 150 amino acid recombinant protein has a predicted molecular mass of 17 kDa. The DTT-reduced protein migrates in the range of 18 to 25 kDa by SDS-PAGE.
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途径
- JAK/STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
抗原
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