PKM Protein (AA 2-531) (His tag)
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- 抗原 See all PKM 蛋白
- PKM (Pyruvate Kinase M1/2 (PKM))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-531
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This PKM protein is labelled with His tag.
- 原理
- Recombinant Human Pyruvate Kinase M2/PKM2 (N-6His)
- 序列
- MGSSHHHHHH SSGLVPRGSH MSKPHSEAGT AFIQTQQLHA AMADTFLEHM CRLDIDSPPI TARNTGIICT IGPASRSVET LKEMIKSGMN VARLNFSHGT HEYHAETIKN VRTATESFAS DPILYRPVAV ALDTKGPEIR TGLIKGSGTA EVELKKGATL KITLDNAYME KCDENILWLD YKNICKVVEV GSKIYVDDGL ISLQVKQKGA DFLVTEVENG GSLGSKKGVN LPGAAVDLPA VSEKDIQDLK FGVEQDVDMV FASFIRKASD VHEVRKVLGE KGKNIKIISK IENHEGVRRF DEILEASDGI MVARGDLGIE IPAEKVFLAQ KMMIGRCNRA GKPVICATQM LESMIKKPRP TRAEGSDVAN AVLDGADCIM LSGETAKGDY PLEAVRMQHL IAREAEAAIY HLQLFEELRR LAPITSDPTE ATAVGAVEAS FKCCSGAIIV LTKSGRSAHQ VARYRPRAPI IAVTRNPQTA RQAHLYRGIF PVLCKDPVQE AWAEDVDLRV NFAMNVGKAR GFFKKGDVVI VLTGWRPGSG FTNTMRVVPV P
- 产品特性
- Recombinant Human PKM2 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Pro531) of Human PKM2 fused with a 6His tag at the C-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product PKM 蛋白
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Pyruvate Kinase M1/2 (PKM) (AA 2-531) protein (His tag)
Crystallography grade PKM 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Pyruvate Kinase M1/2 (PKM) (AA 2-531) protein (His tag)Crystallography grade PKM 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Pyruvate Kinase M1/2 (PKM) (AA 1-531) (Active) protein (His tag)PKM 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % by SDS - PAGE SDS Active
Pyruvate Kinase M1/2 (PKM) proteinPKM 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, ELISA, IP
Pyruvate Kinase M1/2 (PKM) proteinPKM 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, ELISA, IP
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Supplied as a 0.2 μm filtered solution of PBS, pH 7.0, 10 % glycerol.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- -80 °C
- 储存方法
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - 有效期
- 6 months
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- 抗原
- PKM (Pyruvate Kinase M1/2 (PKM))
- 别名
- Pyruvate Kinase PKM (PKM 产品)
- 别名
- CTHBP Protein, OIP3 Protein, PK3 Protein, PKM2 Protein, TCB Protein, THBP1 Protein, AA414905 Protein, AL024370 Protein, AL024424 Protein, Pk-2 Protein, Pk-3 Protein, Pk3 Protein, Pkm2 Protein, PKM12 Protein, CG7070 Protein, Dmel\\CG7070 Protein, PYK Protein, Pyk Protein, cb1029 Protein, fj33f03 Protein, pkm2 Protein, pkm2a Protein, wu:fj33f03 Protein, MGC53388 Protein, PKM Protein, PK-S Protein, pk3 Protein, pkm2b Protein, zgc:92037 Protein, pyruvate kinase M1/2 Protein, pyruvate kinase, muscle Protein, Pyruvate kinase Protein, pyruvate kinase M1/2a Protein, pyruvate kinase PKM Protein, pyruvate kinase (PK) Protein, pyruvate kinase Protein, pyruvate kinase M1/2 S homeolog Protein, pyruvate kinase M1/2b Protein, PKM Protein, Pkm Protein, PyK Protein, pkma Protein, pkm Protein, LOC710798 Protein, pyk Protein, pk Protein, LOC100534543 Protein, pkm.S Protein, pkmb Protein
- 背景
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Pyruvate kinase PKM is an isoenzyme of the glycolytic enzyme pyruvate kinase. Depending upon the different metabolic functions of the tissues, different isoenzymes of pyruvate kinase are expressed. The pyruvate kinase isoenzyme type M2(PKM2) is expressed in some differentiated tissues, such as lung, fat tissue, retina, and pancreatic islets, as well as in all cells with a high rate of nucleic acid synthesis, such as normal proliferating cells, embryonic cells, and especially tumor cells. As a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation and Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Synonyms: Cytosolic thyroid hormone-binding protein,Opa-interacting protein 3,Pyruvate kinase 2/3,Pyruvate kinase muscle isozyme,Thyroid hormone-binding protein 1,Tumor M2-PK,p58,CTHBP,OIP3,PK2,PK3,TCB,THBP1 - 分子量
- 60.1 kDa
- UniProt
- P14618
- 途径
- Warburg Effect
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