AFT1 Protein (AA 1-271) (His tag)
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- 抗原 See all AFT1 蛋白
- AFT1 (Activating Transcription Factor 1 (AFT1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-271
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This AFT1 protein is labelled with His tag.
- 原理
- Recombinant Human Activating Transcription Factor 1/ATF1 (C-6His)
- 序列
- MEDSHKSTTS ETAPQPGSAV QGAHISHIAQ QVSSLSESEE SQDSSDSIGS SQKAHGILAR RPSYRKILKD LSSEDTRGRK GDGENSGVSA AVTSMSVPTP IYQTSSGQYI AIAPNGALQL ASPGTDGVQG LQTLTMTNSG STQQGTTILQ YAQTSDGQQI LVPSNQVVVQ TASGDMQTYQ IRTTPSATSL PQTVVMTSPV TLTSQTTKTD DPQLKREIRL MKNREAAREC RRKKKEYVKC LENRVAVLEN QNKTLIEELK TLKDLYSNKS VVEHHHHHH
- 产品特性
- Recombinant Human Cyclic AMP-dependent transcription factor ATF-1 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Val271) of Human ATF1 fused with a 6His tag at the C-terminus..
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product AFT1 蛋白
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Activating Transcription Factor 1 (AFT1) (AA 1-271) protein (GST tag)
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Activating Transcription Factor 1 (AFT1) (AA 1-269) protein (Strep Tag)Crystallography grade AFT1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Activating Transcription Factor 1 (AFT1) proteinAFT1 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, ELISA, IP
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- AFT1 (Activating Transcription Factor 1 (AFT1))
- 别名
- ATF1 (AFT1 产品)
- 别名
- ews-atf1 Protein, fus/atf-1 Protein, treb36 Protein, ATF1 Protein, EWS-ATF1 Protein, FUS/ATF-1 Protein, TREB36 Protein, Atf-1 Protein, wu:fc92c07 Protein, zgc:64099 Protein, activating transcription factor 1 Protein, activating transcription factor 1 L homeolog Protein, atf1 Protein, ATF1 Protein, MGC85066 Protein, atf1.L Protein, Atf1 Protein
- 背景
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Cyclic AMP-dependent transcription factor ATF-1(ATF1) which contains 1 bZIP (basic-leucine zipper) domain and 1 KID (kinase-inducible) domain, belongs to the bZIP family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. ATF1 binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It also binds to the Tax-responsive element (TRE) of HTLV-I. ATF1 mediates PKA-induced stimulation of CRE-reporter genes, represses the expression of FTH1 and other antioxidant detoxification genes, triggers cell proliferation and transformation. ATF1 is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and CDK3. Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation.
Synonyms: Cyclic AMP-dependent transcription factor ATF-1,cAMP-dependent transcription factor ATF-1,Activating transcription factor 1, - 分子量
- 30.3 kDa
- UniProt
- P18846
- 途径
- Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades
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