Sonic Hedgehog Protein (SHH) (AA 25-198, Cys25IleIle-Mutant)
Quick Overview for Sonic Hedgehog Protein (SHH) (AA 25-198, Cys25IleIle-Mutant) (ABIN2018373)
抗原
See all Sonic Hedgehog (SHH) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 25-198, Cys25IleIle-Mutant
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产品特性
- ED50<1.0 μg/mL, measured by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) Cells, corresponding to a specific activity of>1.0× 10^3 units/mg.
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内毒素水平
- < 0.2 EU/μg, determined by LAL method.
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Sonic Hedgehog (SHH) (AA 24-197), (Cys24IleIle-Mutant) (Active) protein
SHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Sonic Hedgehog (SHH) (AA 24-197) proteinSHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sonic Hedgehog (SHH) protein (His tag)SHH 宿主: 小鼠 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Sonic Hedgehog (SHH) (Active) proteinSHH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
Sonic Hedgehog (SHH) (AA 25-198) protein (His tag)SHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE SDS
Sonic Hedgehog (SHH) (AA 24-197) protein (His tag)SHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Sonic Hedgehog (SHH) (AA 181-280) protein (GST tag)SHH 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sonic Hedgehog (SHH) protein (His tag)SHH 宿主: 人 宿主: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Sonic Hedgehog (SHH) (Active) protein (His tag)SHH 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
Sonic Hedgehog (SHH) proteinSHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstituted in ddH2O at 100 μg/mL.
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缓冲液
- Lyophilized after extensive dialysis against PBS.
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储存条件
- -80 °C
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储存方法
- Lyophilized recombinant mouseSonic Hedgehog(rmShh)remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmShhshould be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
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有效期
- 6 months
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- Sonic Hedgehog (SHH)
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背景
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Sonic Hedgehog (Shh) is a member of the Hedgehog (Hh) family of highly conserved proteins which are widely represented throughout the animal kingdom. In mammal, there are threerelatedHh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh). They share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93 % identical). Sonic Hedgehog plays a role in cell growth, cell specialization, and the normal shaping (patterning) of the body. Shh is also important for development of the brain and spinal cord (central nervous system), eyes, limbs, and many other parts of the body. Recombinant mouseSonic Hedgehog(rmShh) produced in E.coli is a single non-glycosylated polypeptide chain containing 176 amino acids. A fully biologically active molecule, rmShh,is obtained by proprietary chromatographic techniques with a molecular mass of 19.8 kDa analyzed by reducing SDS-PAGE.
Synonyms: Hhg1 -
分子量
- 19.8kDa, observed by reducing SDS-PAGE.
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UniProt
- Q62226
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途径
- Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
抗原
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