AA 1-305 (Lys82Asn), expressed with a C-terminal TAT peptide.
纯度
> 95 % by SDS-PAGE and HPLC analyses.
过滤
Sterile filtered
内毒素水平
< 0.2 EU/μg, determined by LAL method.
限制
仅限研究用
状态
Liquid
缓冲液
Sterile Filtered solution contains 10 mM PB, 300 mM NaCl, pH 7.4.
注意事项
Avoid repeated freezing and thawing cycles.
储存条件
4 °C/-20 °C
储存方法
Recombinant human NANOG-TAT (rhNANOG-TAT) remains stable up to 1-2 weeks at 4 °C from date of receipt. For long term storage, aliquot and store at -20 °C or -80 °C.
抗原
Nanog-TAT
背景
NANOG is a transcription factor involved with self-renewal of inner cell mass and embryonic stem (ES) cells by functioning in concert with other factors such as POU5F1 (Oct-4) and SOX2. Nanog imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages, and blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes.Recombinant human NANOG-TAT (rhNANOG-TAT) produced in E. coli is a single chain, 318 amino acids non-glycosylated polypeptide. A fully biologically active molecule, rhNANOG-TAT has a molecular mass of 36.2 kDa analyzed by reducing SDS-PAGE. Synonyms: None