The EC50 value of human MPIF-1/CCL23 on Ca2+ mobilization assay in CHO-K1/Ga15/hCCR1 cells (human Ga15 and human CCR1 stably expressed in CHO-K1 cells) is less than 2 μg/mL. Alpha chain: AA 32-494, beta chain: AA 495-728
CCL23
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
Greater than 95 % as determined by reducing SDS-PAGE.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstituted in ddH2O or PBS at 100 μg/mL.
缓冲液
Lyophilized after extensive dialysis against PBS.
储存条件
-80 °C
储存方法
Lyophilized recombinant humanMPIF-1/CCL23 remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, human MPIF-1/CCL23 should be stable up to 1 week at 4 °C or up to 2 months at -20 °C.
Myeloid progenitor inhibitory factor 1 (MPIF-1), also known as Chemokine (C-C motif) ligand 23 (CCL23) is a small cytokine belonging to the CC chemokine family.MPIF-1is predominantly expressed in lung and liver tissue, but is also found in bone marrow and placenta. It is also expressed in some cell lines of myeloid origin. It is highly chemotactic for resting T cells and monocytes and slightly chemotactic for neutrophils. MPIF-1 has been shown to inhibit colony formation of bone marrow myeloid immature progenitors. It has also been attributed to an inhibitory activity on hematopoietic progenitor cells. MPIF-1 is a ligand for the chemokine receptor CCR1.Recombinant human MPIF-1/CCL23 produced in CHO cells is a single polypeptide chain containing 99 amino acids. A fully biologically active molecule, rhMPIF-1/CCL23 has a molecular mass of 12 kDa analyzed by reducing SDS-PAGE. Synonyms: MPIF-1/CCL23, Human