Recombinant Human Ubiquitin-Conjugating Enzyme E2 R2/UBE2R2 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Ser238) of Human UBE2R2 fused with a His tag at the N-terminus.
纯度
> 95 % as determined by reducing SDS-PAGE.
过滤
0.2 μm filtered
内毒素水平
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
缓冲液
Supplied as a 0.2 μm filtered solution of 50mm HEPES,150 mM NaCl, pH 7.5.
注意事项
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
储存条件
-80 °C
储存方法
Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
Ubiquitin-Conjugating Enzyme E2 R2 (UBE2R2) is a modification enzyme that belongs to the ubiquitin-conjugating enzyme family. UBE2R2 is involved in cell growth and transformation. It accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, UBE2R2 catalyzes monoubiquitination and 'Lys-48'-linked polyubiquitination. It may be involved in degradation of katenin. Alternative Names: Ubiquitin-Conjugating Enzyme E2 R2, Ubiquitin Carrier Protein R2, Ubiquitin-Conjugating Enzyme E2-CDC34B, Ubiquitin-Protein Ligase R2, UBE2R2, CDC34B, UBC3B