Recombinant Human D-Beta-Hydroxybutyrate Dehydrogenase Mitochondrial/BDH1 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Arg343) of Human BDH1 fused with a 6His tag at the N-terminus.
纯度
> 95 % as determined by reducing SDS-PAGE.
过滤
0.2 μm filtered
内毒素水平
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
缓冲液
Supplied as a 0.2 μm filtered solution of 50 mM Tris, 1 mM DTT, pH 7.5.
储存液
Dithiothreitol (DTT)
注意事项
This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
储存条件
-80 °C
储存方法
Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
有效期
6 months
抗原
BDH1
(3-hydroxybutyrate Dehydrogenase, Type 1 (BDH1))
zgc:163053 Protein, BDH Protein, SDR9C1 Protein, 2310032J20Rik Protein, AI327223 Protein, Bdh Protein, 3-hydroxybutyrate dehydrogenase 1 Protein, 3-hydroxybutyrate dehydrogenase 1 S homeolog Protein, 3-hydroxybutyrate dehydrogenase, type 1 Protein, 3-hydroxybutyrate dehydrogenase, type 1 A Protein, BDH1 Protein, bdh1.S Protein, bdh1 Protein, Bdh1 Protein, BDH1A Protein
Sub Type
Fusionprotein
背景
D-Beta-Hydroxybutyrate Dehydrogenase Mitochondrial (BDH) is a member of the short-chain dehydrogenases/reductases (SDR) family. BDH is localized in the mitochondrion matrix. BDH forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific necessity for phosphatidylcholine for optimal enzymatic activity. BDH catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the 2 main ketone bodies formed during fatty acid catabolism. Alternative Names: D-Beta-Hydroxybutyrate Dehydrogenase Mitochondrial, BDH, 3-Hydroxybutyrate Dehydrogenase, BDH