CGREF1 Protein (AA 20-301) (His tag)
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- 抗原 See all CGREF1 products
- CGREF1 (Cell Growth Regulator with EF-Hand Domain 1 (CGREF1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 20-301
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宿主
- 人
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资源
- 人细胞
- 标记
- This CGREF1 protein is labelled with His tag.
- 原理
- Recombinant Human CGREF1/CGR11 (C-6His)
- 序列
- APKDGVTRPD SEVQHQLLPN PFQPGQEQLG LLQSYLKGLG RTEVQLEHLS REQVLLYLFA LHDYDQSGQL DGLELLSMLT AALAPGAANS PTTNPVILIV DKVLETQDLN GDGLMTPAEL INFPGVALRH VEPGEPLAPS PQEPQAVGRQ SLLAKSPLRQ ETQEAPGPRE EAKGQVEARR ESLDPVQEPG GQAEADGDVP GPRGEAEGQA EAKGDAPGPR GEAGGQAEAE GDAPGPRGEA GGQAEARENG EEAKELPGET LESKNTQNDF EVHIVQVEND EIVDHHHHHH
- 产品特性
- Recombinant Human Cell Growth Regulator with EF Hand Domain Protein 1/CGREF1 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Ala20-Ile301) of Human CGREF1 fused with a polyhistidine tag at the C-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Cell Growth Regulator with EF-Hand Domain 1 (CGREF1) (AA 20-301) protein (His tag)
CGREF1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Cell Growth Regulator with EF-Hand Domain 1 (CGREF1) (AA 136-235) protein (GST tag)CGREF1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Cell Growth Regulator with EF-Hand Domain 1 (CGREF1) protein (Myc-DYKDDDDK Tag)CGREF1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl,1 mM GaCl2, pH 7.5.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - 有效期
- 3 months
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- 抗原
- CGREF1 (Cell Growth Regulator with EF-Hand Domain 1 (CGREF1))
- 别名
- CGREF1 (CGREF1 产品)
- 别名
- CGR11 Protein, CGREF1 Protein, 1110004G24Rik Protein, AW045201 Protein, Cgr11 Protein, cell growth regulator with EF-hand domain 1 Protein, cell growth regulator with EF-hand domain 1 L homeolog Protein, cell growth regulator with ring finger domain 1 Protein, cell growth regulator with EF hand domain 1 Protein, CGREF1 Protein, cgref1.L Protein, cgref1 Protein, CGRRF1 Protein, Cgref1 Protein
- Sub Type
- Fusionprotein
- 背景
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Cell Growth Regulator with EF Hand Domain Protein 1 (CGREF1) is a secreted calcium ion binding protein. CGREF1 contains two EF-hand domains and both EF-hands are required for function. Human CGREF1 is synthesized as a 301 amino acid precursor that contains a 19 amino acid signal sequence, and a 282 amino acid mature chain. CGREF1 is probably digested extracellularly by an unknown serine protease generating extremely hydrophobic bioactive peptides. CGREF1 mediates cell-cell adhesion in a calcium-dependent manner. In addition, CGREF1 is able to inhibit growth in several cell lines.
Alternative Names: Cell Growth Regulator with EF Hand Domain Protein 1, Cell Growth Regulatory Gene 11 Protein, Hydrophobestin, CGREF1, CGR11 - 分子量
- 30.9 kDa
- UniProt
- Q99674
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