Recombinant Human High Mobility Group Protein B2/HMGB2 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Gly2-Glu209) of Human HMGB2 fused with a 6His tag at the C-terminus.
纯度
> 95 % as determined by reducing SDS-PAGE.
过滤
0.2 μm filtered
内毒素水平
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
缓冲液
Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.2.
注意事项
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
储存条件
4 °C/-20 °C/-80 °C
储存方法
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Sub Type
Fusionprotein
背景
High Mobility Group Protein B2 (HMGB2) belongs to the non-histone chromosomal high-mobility group protein family. Members of this family are chromatin-associated and widely spread in the nucleus of higher eukaryotic cells. HMGB2 contains 2 HMG box DNA-binding domains. It is associated with chromatin and has the ability to bend DNA, preferentially single-stranded DNA. It is shown that HMGB2 is able to efficiently bend DNA and form DNA circles. In addition, HMGB2 is involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. Alternative Names: High Mobility Group Protein B2, High Mobility Group Protein 2, HMG-2, HMGB2, HMG2