抗原 See all NLRP3 蛋白: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))
蛋白类型: Recombinant
产品特性: AA 1-1036
宿主 5 2: 人
资源 3 2 1 1: 小麦胚
标记: This NLRP3 protein is labelled with GST tag.
应用范围: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
原理: NLRP3 (Human) Recombinant Protein (P01)
序列: MKMASTRCKL ARYLEDLEDV DLKKFKMHLE DYPPQKGCIP LPRGQTEKAD HVDLATLMID FNGEEKAWAM AVWIFAAINR RDLYEKAKRD EPKWGSDNAR VSNPTVICQE DSIEEEWMGL LEYLSRISIC KMKKDYRKKY RKYVRSRFQC IEDRNARLGE SVSLNKRYTR LRLIKEHRSQ QEREQELLAI GKTKTCESPV SPIKMELLFD PDDEHSEPVH TVVFQGAAGI GKTILARKMM LDWASGTLYQ DRFDYLFYIH CREVSLVTQR SLGDLIMSCC PDPNPPIHKI VRKPSRILFL MDGFDELQGA FDEHIGPLCT DWQKAERGDI LLSSLIRKKL LPEASLLITT RPVALEKLQH LLDHPRHVEI LGFSEAKRKE YFFKYFSDEA QARAAFSLIQ ENEVLFTMCF IPLVCWIVCT GLKQQMESGK SLAQTSKTTT AVYVFFLSSL LQPRGGSQEH GLCAHLWGLC SLAADGIWNQ KILFEESDLR NHGLQKADVS AFLRMNLFQK EVDCEKFYSF IHMTFQEFFA AMYYLLEEEK EGRTNVPGSR LKLPSRDVTV LLENYGKFEK GYLIFVVRFL FGLVNQERTS YLEKKLSCKI SQQIRLELLK WIEVKAKAKK LQIQPSQLEL FYCLYEMQEE DFVQRAMDYF PKIEINLSTR MDHMVSSFCI ENCHRVESLS LGFLHNMPKE EEEEEKEGRH LDMVQCVLPS SSHAACSHGL VNSHLTSSFC RGLFSVLSTS QSLTELDLSD NSLGDPGMRV LCETLQHPGC NIRRLWLGRC GLSHECCFDI SLVLSSNQKL VELDLSDNAL GDFGIRLLCV GLKHLLCNLK KLWLVSCCLT SACCQDLASV LSTSHSLTRL YVGENALGDS GVAILCEKAK NPQCNLQKLG LVNSGLTSVC CSALSSVLST NQNLTHLYLR GNTLGDKGIK LLCEGLLHPD CKLQVLELDN CNLTSHCCWD LSTLLTSSQS LRKLSLGNND LGDLGVMMFC EVLKQQSCLL QNLGLSEMYF NYETKSALET LQEEKPELTV VFEPSW
产品特性: Human NLRP3 full-length ORF ( NP_004886.3, 1 a.a. - 1036 a.a.) recombinant protein with GST-tag at N-terminal.
纯化方法: in vitro wheat germ expression system

NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 5-218) protein (His-IF2DI Tag)

NLRP3 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA

NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 1-100) protein (GST tag)

NLRP3 宿主: 人宿主: 小麦胚 Recombinant WB, ELISA, AP, AA

NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 1-1036) protein (Strep Tag)

Crystallography grade NLRP3 宿主: 人宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA

NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 1-1033) protein (Strep Tag)

Crystallography grade NLRP3 宿主: 小鼠宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA

NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 671-1033) protein (His tag)

NLRP3 宿主: 小鼠宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, Imm, PC

NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 770-1036) protein (His tag)

NLRP3 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant > 90 % WB, SDS, Imm, PC

NLR Family, Pyrin Domain Containing 3 (NLRP3) protein (DYKDDDDK Tag)

NLRP3 宿主: 人宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

应用备注: Optimal working dilution should be determined by the investigator.
说明: Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.
限制: 仅限研究用

缓冲液: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
注意事项: Aliquot to avoid repeated freezing and thawing.
储存条件: -80 °C
储存方法: Best use within three months from the date of receipt of this protein.

Shi, Wang, Li, Zhan, Tang, Fina, Su, Pratt, Bu, Hildebrand, Lyon, Scott, Quan, Sun, Russell, Arnett, Jurek, Chen, Kravchenko, Mathison, Moresco, Monson, Ulevitch, Beutler: "NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component." in: Nature immunology, Vol. 17, Issue 3, pp. 250-8, (2016) (PubMed).

He, Varadarajan, Muñoz-Planillo, Burberry, Nakamura, Núñez: "3,4-methylenedioxy-?-nitrostyrene inhibits NLRP3 inflammasome activation by blocking assembly of the inflammasome." in: The Journal of biological chemistry, Vol. 289, Issue 2, pp. 1142-50, (2014) (PubMed).

Cocco, Garella, Di Stilo, Borretto, Stevanato, Giorgis, Marini, Fantozzi, Miglio, Bertinaria: "Electrophilic warhead-based design of compounds preventing NLRP3 inflammasome-dependent pyroptosis." in: Journal of medicinal chemistry, Vol. 57, Issue 24, pp. 10366-82, (2014) (PubMed).

Bruchard, Mignot, Derangère, Chalmin, Chevriaux, Végran, Boireau, Simon, Ryffel, Connat, Kanellopoulos, Martin, Rébé, Apetoh, Ghiringhelli: "Chemotherapy-triggered cathepsin B release in myeloid-derived suppressor cells activates the Nlrp3 inflammasome and promotes tumor growth." in: Nature medicine, Vol. 19, Issue 1, pp. 57-64, (2013) (PubMed).

Lu, Nakamura, Inouye, Li, Tang, Lundbäck, Valdes-Ferrer, Olofsson, Kalb, Roth, Zou, Erlandsson-Harris, Yang, Ting, Wang, Andersson, Antoine, Chavan, Hotamisligil, Tracey: "Novel role of PKR in inflammasome activation and HMGB1 release." in: Nature, Vol. 488, Issue 7413, pp. 670-4, (2012) (PubMed).

抗原: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))
别名: NLRP3 (NLRP3 产品)
别名: CIAS1 Protein, Nalp3 Protein, avp Protein, cias1 Protein, nalp3 Protein, pypaf1 Protein, agtavprl Protein, AGTAVPRL Protein, AII Protein, AVP Protein, C1orf7 Protein, CLR1.1 Protein, FCAS Protein, FCU Protein, MWS Protein, NALP3 Protein, PYPAF1 Protein, AII/AVP Protein, Cias1 Protein, Mmig1 Protein, Pypaf1 Protein, NLR family pyrin domain containing-like Protein, NLR family pyrin domain containing 3 Protein, NLR family, pyrin domain containing 3 L homeolog Protein, NLR family, pyrin domain containing 3 Protein, NLRPL Protein, NLRP3 Protein, nlrp3.L Protein, Nlrp3 Protein
背景: Full Gene Name: NLR family, pyrin domain containing 3
Synonyms: AGTAVPRL,AII,AII/AVP,AVP,C1orf7,CIAS1,CLR1.1,FCAS,FCU,FLJ95925,MWS,NALP3,PYPAF1
基因ID: 114548
NCBI登录号: NM_004895
途径: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome

NLRP3 Protein (AA 1-1036) (GST tag)

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