(4 references)-
- 抗原 See all GM-CSF (CSF2) 蛋白
- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 大鼠
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- ELISA, Functional Studies (Func)
- 品牌
- BD Pharmingen™
- 特异性
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Biological Activity Range: Measured in a proliferation assay using MC/9 indicator cells
Specific Activity: 0.1 - 1.0 X 10^9 Units /mg
ED50: 10 - 100 pg/ml
Observed dose response: 100 fold A Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation. Since applications vary, each investigator should titrate the reagent to obtain optimal results. - 产品特性
- Recombinant rat GM-CSF is supplied as a frozen liquid comprised of 0.22 µm sterile filtered aqueous buffered solution and containing 1.0 mg/ml Biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Recombinant rat GM-CSF is purified by immunoaffinity chromatography and was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law.
- 纯化方法
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Immunoaffinity Chromatography
Recombinant rat GM-CSF is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, containing bovine serum albumin and glycerol, with no preservatives. Recombinant rat GM-CSF is ≥ 95 % pure as determined by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert Law. - 纯度
- ≥ 95 %
- 过滤
- 0.22 μm filtered
- 内毒素水平
- The endotoxin level is ≤ 0.1 ng per µg of rat GM-CSF, as measured in chromogenic LAL assay.
- Top Product
- Discover our top product CSF2 蛋白
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Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) protein
CSF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 8-144) proteinCSF2 宿主: 人 宿主: Tobacco (Nicotiana benthamiana) Recombinant WB, CC
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (Active) protein (His tag)CSF2 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (Active) proteinCSF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-141) (Active) proteinCSF2 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) protein (His tag)CSF2 宿主: 大鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 1-144) protein (GST tag)CSF2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) (Active) protein (His tag)CSF2 宿主: 人 宿主: HEK-293 Cells > 95 % CC, AcA Active
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- 应用备注
- ELISA Standard: Recombinant rat GM-CSF is useful as a quantitative standard for measuring rat GM-CSF protein levels in a GM-CSF specific sandwich ELISA with the purified B61-5/B61-9 antibody cocktail as a capture reagent and the biotinylated monoclonal B61-10 antibody as the detection antibody.
- 说明
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BD Pharmingen™ Recombinant Rat GM-CSF - Reactivity Rat
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.1 mg/mL
- 缓冲液
- Frozen aqueous buffered solution containing BSA and glycerol.
- 储存液
- Azide free
- 注意事项
- Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is a cytokine which is made by activated T cells, macrophages, vascular endothelial cells and fibroblasts. It has potent stimulatory effects on the growth and differentiation of bone marrow progenitor cells that generate granulocytes monocytes/macrophages, and megakaryocytes. In peripheral tissues, GM-CSF can act on mature leukocytes to promote inflammatory responses.
- 储存条件
- -80 °C
- 储存方法
- Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "Interleukin-3 and encephalitogenic activity of SJL/J myelin basic protein-specific T cell lines." in: Journal of neuroimmunology, Vol. 43, Issue 1-2, pp. 69-78, (1993) (PubMed).
: "Molecular physiology of granulocyte-macrophage colony-stimulating factor." in: Blood, Vol. 77, Issue 6, pp. 1131-45, (1991) (PubMed).
: "Structure of the chromosomal gene for granulocyte-macrophage colony stimulating factor: comparison of the mouse and human genes." in: The EMBO journal, Vol. 4, Issue 10, pp. 2561-8, (1985) (PubMed).
: "Molecular cloning of cDNA encoding a murine haematopoietic growth regulator, granulocyte-macrophage colony stimulating factor." in: Nature, Vol. 309, Issue 5971, pp. 763-7, (1984) (PubMed).
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: "Interleukin-3 and encephalitogenic activity of SJL/J myelin basic protein-specific T cell lines." in: Journal of neuroimmunology, Vol. 43, Issue 1-2, pp. 69-78, (1993) (PubMed).
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- 抗原
- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
- 别名
- GM-CSF (CSF2 产品)
- 别名
- GMCSF Protein, GM-CSF Protein, CSF2 Protein, Csfgm Protein, Gm-CSf Protein, MGI-IGM Protein, Gm-csf Protein, Gmcsf Protein, CSF Protein, colony stimulating factor 2 Protein, colony stimulating factor 2 (granulocyte-macrophage) Protein, CSF2 Protein, Csf2 Protein
- 背景
- Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is a cytokine which is made by activated T cells, macrophages, vascular endothelial cells and fibroblasts. It has potent stimulatory effects on the growth and differentiation of bone marrow progenitor cells that generate granulocytes monocytes/macrophages, and megakaryocytes. In peripheral tissues, GM-CSF can act on mature leukocytes to promote inflammatory responses.
- 途径
- JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin
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