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IL12 蛋白

Name: IL12 蛋白
SKU: ABIN1305066
Availability: OutOfStock

This Recombinant IL12 protein is expressed in 大肠杆菌（E. Coli） and has been mentioned in 1 publication.

(1 reference)

产品编号 ABIN1305066

发货至: 中国

Datasheet as PDF

Quick Overview for IL12 蛋白 (ABIN1305066)

抗原

See all IL12 蛋白

IL12 (Interleukin 12 (IL12))

蛋白类型

Recombinant

生物活性

Active

宿主

小鼠

资源

大肠杆菌（E. Coli）

应用范围

ELISA, Functional Studies (Func)

纯度

≥ 95 %

Discover our top product IL12 蛋白

品牌

BD Pharmingen™

特异性

Biological Activity Range: Measured by 2D6 cell proliferation Specific Activity: 0.2 - 2.0 x 10^9 Units /mg
ED50: 5 - 50 pg/ml
Observed dose response: > 200 fold
Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation

产品特性

The recombinant mouse IL-12 was lyophilized in a solution comprised of 0.22 µm sterile-filtered aqueous buffered solution containing 2.0 mg/ml Biotechnology grade, low endotoxin bovine serum albumin, and containing no preservatives. The mass ratio of carrier-protein to mouse IL-12 is 40:1 or 40 µg BSA to 1 µg IL-12. Recombinant mouse IL-12 was found to be > 95% pure by SDS-PAGE and an absorbance assay based on the Beer-Lambert law.

纯化方法

Recombinant mouse IL-12 p70 is provided lyophilized, containing bovine serum albumin and no preservatives. Recombinant mouse IL-12 p70 was found to be ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beer-Lambert law.

过滤

0.22 μm filtered

内毒素水平

The endotoxin level is ≤ 0.1 ng per µg of mouse IL-12, as measured in a chromogenic LAL assay.
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Interleukin 12 (IL12) (AA 23-328) protein (His-Avi Tag)
IL12 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC

Interleukin 12 (IL12) (AA 23-328) protein (His-Avi Tag,Biotin)
IL12 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Interleukin 12 (IL12) (AA 23-219), (AA 23-328) (Active) protein
IL12 宿主: 人宿主: HEK-293T Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active

Interleukin 12 (IL12) (AA 23-215) protein (His tag)
IL12 宿主: 小鼠宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Interleukin 12 (IL12) (AA 23-328) (Active) protein (His tag,DYKDDDDK Tag)
IL12 宿主: 人宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active

Interleukin 12 (IL12) (AA 23-328) (Active) protein (His tag,AVI tag,DYKDDDDK Tag,Biotin)
IL12 宿主: 人宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active

Interleukin 12 (IL12) (Active) protein (His tag)
IL12 宿主: 人宿主: Baculovirus infected Insect Cells Recombinant > 90% by SDS-PAGE SDS, AcA Active

Interleukin 12 (IL12) protein
IL12 宿主: 小鼠宿主: 人细胞 Recombinant > 90 % as determined by SDS-PAGE

Interleukin 12 (IL12) protein (His tag)
IL12 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.

Interleukin 12 (IL12) (AA 23-219) protein (Fc-His Tag)
IL12 宿主: 人宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

应用备注

ELISA Standard: This mouse IL-12 is useful as a quantitative standard for measuring mouse IL-12 protein levels in an IL-12 specific sandwich ELISA with the purified 9A5 antibody (Cat. No. 554658) as a capture antibody and the biotinylated C17.8 (Cat. No. 554476) as the detection antibody. To obtain linear standard curves, doubling dilutions of this mouse IL-12 standard from 4000-30 pg/ml should be included in each ELISA plate.

说明

BD Pharmingen™ Recombinant Mouse IL-12 p70 - Purified - Clone NA - Reactivity Ms

限制

仅限研究用
状态

Lyophilized

缓冲液

Lyophilized in an aqueous buffered solution containing BSA.

注意事项

Interleukin-12 (IL-12) is a potent regulator of cell-mediated immune responses. Biologically active IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD (p35) and 40 kD (p40) subunits. IL-12 binds to specific high affinity receptors and can, i) stimulate the growth of activated CD4+ and CD8+ T cells and natural killer (NK) cells, ii) promote the development of proinflammatory/Th1-like CD4+ cells and cytotoxic CD8+ T cells, iii) increase the lytic activity of NK cells, lymphokine-activated killer cells and antibody dependent cell-mediated cytotoxic cells, and, iv) induce NK and T cells to secrete large amounts of the proinflammatory cytokine, IFN-γ.

储存条件

-80 °C

储存方法

Store product at -80°C prior to use or for long term storage of stock solutions. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Schoenhaut, Chua, Wolitzky, Quinn, Dwyer, McComas, Familletti, Gately, Gubler: "Cloning and expression of murine IL-12." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 11, pp. 3433-40, (1992) (PubMed).
抗原

IL12 (Interleukin 12 (IL12))

别名

IL-12 (p70)

背景

Interleukin-12 (IL-12) is a potent regulator of cell-mediated immune responses. Biologically active IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD (p35) and 40 kD (p40) subunits. IL-12 binds to specific high affinity receptors and can, i) stimulate the growth of activated CD4+ and CD8+ T cells and natural killer (NK) cells, ii) promote the development of proinflammatory/Th1-like CD4+ cells and cytotoxic CD8+ T cells, iii) increase the lytic activity of NK cells, lymphokine-activated killer cells and antibody dependent cell-mediated cytotoxic cells, and, iv) induce NK and T cells to secrete large amounts of the proinflammatory cytokine, IFN-γ.

途径

JAK/STAT Signaling, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome