IL12 蛋白
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北京 101111
Quick Overview for IL12 蛋白 (ABIN1305066)
抗原
See all IL12 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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特异性
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Biological Activity Range: Measured by 2D6 cell proliferation Specific Activity: 0.2 - 2.0 x 10^9 Units /mg
ED50: 5 - 50 pg/ml
Observed dose response: > 200 fold
Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation -
产品特性
- The recombinant mouse IL-12 was lyophilized in a solution comprised of 0.22 µm sterile-filtered aqueous buffered solution containing 2.0 mg/ml Biotechnology grade, low endotoxin bovine serum albumin, and containing no preservatives. The mass ratio of carrier-protein to mouse IL-12 is 40:1 or 40 µg BSA to 1 µg IL-12. Recombinant mouse IL-12 was found to be > 95% pure by SDS-PAGE and an absorbance assay based on the Beer-Lambert law.
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纯化方法
- Recombinant mouse IL-12 p70 is provided lyophilized, containing bovine serum albumin and no preservatives. Recombinant mouse IL-12 p70 was found to be ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beer-Lambert law.
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过滤
- 0.22 μm filtered
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内毒素水平
- The endotoxin level is ≤ 0.1 ng per µg of mouse IL-12, as measured in a chromogenic LAL assay.
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Interleukin 12 (IL12) (AA 23-328) protein (His-Avi Tag)
IL12 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
Interleukin 12 (IL12) (AA 23-328) protein (His-Avi Tag,Biotin)IL12 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 12 (IL12) (AA 23-219), (AA 23-328) (Active) proteinIL12 宿主: 人 宿主: HEK-293T Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 12 (IL12) (AA 23-328) (Active) protein (His tag,DYKDDDDK Tag)IL12 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 12 (IL12) (AA 23-328) (Active) protein (His tag,AVI tag,DYKDDDDK Tag,Biotin)IL12 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 12 (IL12) proteinIL12 宿主: 小鼠 宿主: 人细胞 Recombinant > 90 % as determined by SDS-PAGE
Interleukin 12 (IL12) protein (His tag)IL12 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Interleukin 12 (IL12) (AA 23-219) protein (Fc-His Tag)IL12 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 12 (IL12) (AA 23-328) (Active) protein (His tag)IL12 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % SDS, AcA Active
Interleukin 12 (IL12) proteinIL12 宿主: 人 宿主: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
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应用备注
- ELISA Standard: This mouse IL-12 is useful as a quantitative standard for measuring mouse IL-12 protein levels in an IL-12 specific sandwich ELISA with the purified 9A5 antibody (Cat. No. 554658) as a capture antibody and the biotinylated C17.8 (Cat. No. 554476) as the detection antibody. To obtain linear standard curves, doubling dilutions of this mouse IL-12 standard from 4000-30 pg/ml should be included in each ELISA plate.
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说明
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BD Pharmingen™ Recombinant Mouse IL-12 p70 - Purified - Clone NA - Reactivity Ms
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- Lyophilized in an aqueous buffered solution containing BSA.
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注意事项
- Interleukin-12 (IL-12) is a potent regulator of cell-mediated immune responses. Biologically active IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD (p35) and 40 kD (p40) subunits. IL-12 binds to specific high affinity receptors and can, i) stimulate the growth of activated CD4+ and CD8+ T cells and natural killer (NK) cells, ii) promote the development of proinflammatory/Th1-like CD4+ cells and cytotoxic CD8+ T cells, iii) increase the lytic activity of NK cells, lymphokine-activated killer cells and antibody dependent cell-mediated cytotoxic cells, and, iv) induce NK and T cells to secrete large amounts of the proinflammatory cytokine, IFN-γ.
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储存条件
- -80 °C
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储存方法
- Store product at -80°C prior to use or for long term storage of stock solutions. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "Cloning and expression of murine IL-12." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 11, pp. 3433-40, (1992) (PubMed).
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: "Cloning and expression of murine IL-12." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 11, pp. 3433-40, (1992) (PubMed).
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- IL12 (Interleukin 12 (IL12))
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别名
- IL-12 (p70)
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背景
- Interleukin-12 (IL-12) is a potent regulator of cell-mediated immune responses. Biologically active IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD (p35) and 40 kD (p40) subunits. IL-12 binds to specific high affinity receptors and can, i) stimulate the growth of activated CD4+ and CD8+ T cells and natural killer (NK) cells, ii) promote the development of proinflammatory/Th1-like CD4+ cells and cytotoxic CD8+ T cells, iii) increase the lytic activity of NK cells, lymphokine-activated killer cells and antibody dependent cell-mediated cytotoxic cells, and, iv) induce NK and T cells to secrete large amounts of the proinflammatory cytokine, IFN-γ.
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途径
- JAK/STAT Signaling, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
抗原
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