POLR2J2 Protein (His tag)
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- 抗原 See all POLR2J2 蛋白
- POLR2J2 (Polymerase (RNA) II (DNA Directed) Polypeptide J2 (POLR2J2))
- 蛋白类型
- Recombinant
- 宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This POLR2J2 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 纯化方法
- purified by chromatography
- 纯度
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product POLR2J2 蛋白
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Polymerase (RNA) II (DNA Directed) Polypeptide J2 (POLR2J2) (AA 1-115) protein (His tag)
POLR2J2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Polymerase (RNA) II (DNA Directed) Polypeptide J2 (POLR2J2) (AA 1-116) protein (GST tag)POLR2J2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Polymerase (RNA) II (DNA Directed) Polypeptide J2 (POLR2J2) (AA 1-115) protein (GST tag)POLR2J2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Polymerase (RNA) II (DNA Directed) Polypeptide J2 (POLR2J2) (AA 1-115) protein (Strep Tag)Crystallography grade POLR2J2 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Polymerase (RNA) II (DNA Directed) Polypeptide J2 (POLR2J2) protein (Myc-DYKDDDDK Tag)POLR2J2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 说明
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Synonyms: DNA-directed RNA polymerase II subunit RPB11-b1, HRPB11B, RPB11b1
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.25 mg/ml (determined by Bradford assay)
- 缓冲液
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl, 10% glycerol, 1 MM DTT, 250 mM Imidazole
- 储存条件
- 4 °C
- 储存方法
- Avoid repeated freezing and thawing cycles.
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- 抗原
- POLR2J2 (Polymerase (RNA) II (DNA Directed) Polypeptide J2 (POLR2J2))
- 别名
- POLR2J2 (POLR2J2 产品)
- 别名
- HRPB11B Protein, RPB11b1 Protein, RNA polymerase II subunit J2 Protein, POLR2J2 Protein
- 背景
- POLR2J2 is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo. Recombinant human POLR2J2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 15.5 kDa (138aa) confirmed by MALDI-TOF
- NCBI登录号
- NP_116581
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