POLR2E Protein (His tag)
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- 抗原 See all POLR2E 蛋白
- POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This POLR2E protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 纯化方法
- purified by chromatography
- 纯度
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product POLR2E 蛋白
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Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E) (AA 1-210) protein (GST tag)
POLR2E 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E) (AA 1-210) protein (Strep Tag)Crystallography grade POLR2E 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E) (AA 1-210) protein (Strep Tag)Crystallography grade POLR2E 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E) protein (Myc-DYKDDDDK Tag)POLR2E 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 说明
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Synonyms: DNA-directed RNA polymerases I, II, and III subunit RPABC1, hRPB25, hsRPB5, RPABC1, RPB5, XAP4
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/ml (determined by Bradford assay)
- 缓冲液
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 10% glycerol, 1 MM DTT
- 储存条件
- 4 °C
- 储存方法
- Avoid repeated freezing and thawing cycles.
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- 抗原
- POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))
- 别名
- POLR2E (POLR2E 产品)
- 别名
- MGC68604 Protein, polr2e Protein, wu:fk97g10 Protein, zgc:101098 Protein, 2410021N14Rik Protein, 25kDa Protein, AW208866 Protein, RPB5 Protein, XAP4 Protein, RPABC1 Protein, hRPB25 Protein, hsRPB5 Protein, polymerase (RNA) II (DNA directed) polypeptide E, 25kDa S homeolog Protein, RNA polymerase II subunit E Protein, polymerase (RNA) II (DNA directed) polypeptide E, b Protein, polymerase (RNA) II (DNA directed) polypeptide E, 25kDa Protein, polymerase (RNA) II (DNA directed) polypeptide E Protein, DNA-directed RNA polymerases I, II, and III subunit RPABC1 Protein, polr2e.S Protein, POLR2E Protein, polr2eb Protein, polr2e Protein, Polr2e Protein, rpb-5 Protein
- 背景
- POLR2E, also as known as DNA-directed RNA polymerases I, II, and III subunit RPABC1, belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family. POLR2E is the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. POLR2E is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Recombinant human POLR2E protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 27.1kDa (233aa), confirmed by MALDI-TOF
- NCBI登录号
- NP_002686
- 途径
- Regulatory RNA Pathways
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