POLR2J3 Protein (His tag)
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- 抗原 See all POLR2J3 蛋白
- POLR2J3 (Polymerase (RNA) II (DNA Directed) Polypeptide J3 (POLR2J3))
- 蛋白类型
- Recombinant
- 宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This POLR2J3 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 纯化方法
- purified by chromatography
- 纯度
- > 95 % by SDS - PAGE
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Polymerase (RNA) II (DNA Directed) Polypeptide J3 (POLR2J3) (AA 1-115) protein (Strep Tag)
Crystallography grade POLR2J3 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Polymerase (RNA) II (DNA Directed) Polypeptide J3 (POLR2J3) protein (Myc-DYKDDDDK Tag)POLR2J3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 说明
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Synonyms: DNA-directed RNA polymerase II subunit RPB11-b2, POLR2J2, RPB11b1, RPB11b2
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/ml (determined by Bradford assay)
- 缓冲液
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, 40% glycerol,1 MM DTT
- 储存条件
- 4 °C
- 储存方法
- Avoid repeated freezing and thawing cycles.
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- 抗原
- POLR2J3 (Polymerase (RNA) II (DNA Directed) Polypeptide J3 (POLR2J3))
- 别名
- POLR2J3 (POLR2J3 产品)
- 别名
- POLR2J2 Protein, RPB11b1 Protein, RPB11b2 Protein, RNA polymerase II subunit J3 Protein, POLR2J3 Protein
- 背景
- DNA-directed RNA polymerase II subunit RPB11-b2, also known as POLR2J3, belongs to the eukaryotic RPB11 RNA polymerase subunit family. POLR2J (DNA-directed RNA polymerase II subunit J) exist as three variants POLR2J1 (RPB11-a), POLR2J2 (RPB11-b1), and POLR2J3 (RPB11-b2). POLR2J3 is DNA-dependent RNA polymerases that catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Recombinant human POLR2J3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 15.5 kDa (138aa) confirmed by MALDI-TOF
- NCBI登录号
- NP_001091084
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