UBA5 Protein (His tag)
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- 抗原 See all UBA5 蛋白
- UBA5 (Ubiquitin-Like Modifier Activating Enzyme 5 (UBA5))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌(E. Coli)
- 标记
- This UBA5 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 纯化方法
- purified by chromatography
- 纯度
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product UBA5 蛋白
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- 说明
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Synonyms: Ubiquitin-like modifier-activating enzyme 5, THIFP1, UBE1DC1
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/ml (determined by Bradford assay)
- 缓冲液
- 20 mM Tris-HCl buffer (pH 8.0) containing 1 MM DTT, 10% glycerol, 50 mM NaCl
- 储存条件
- 4 °C
- 储存方法
- Avoid repeated freezing and thawing cycles.
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- 抗原
- UBA5 (Ubiquitin-Like Modifier Activating Enzyme 5 (UBA5))
- 别名
- UBA5 (UBA5 产品)
- 别名
- cb972 Protein, ube1dc1 Protein, UBE1DC1 Protein, THIFP1 Protein, 5730525G14Rik Protein, AW240750 Protein, Ube1dc1 Protein, UBAL Protein, ubiquitin-like modifier activating enzyme 5 Protein, ubiquitin like modifier activating enzyme 5 Protein, ubiquitin-activating enzyme E1-like protein Protein, ubiquitin like modifier activating enzyme 5 S homeolog Protein, uba5 Protein, UBA5 Protein, LOC100270786 Protein, Uba5 Protein, uba5.S Protein
- 背景
- UBA5 (ubiquitin-like modifier activating enzyme 5) belongs to the ubiquitin-activating E1 family and UBA5 subfamily. Ubiquitin and ubiquitin-like proteins are known to be covalently conjugated to a variety of cellular substrates via a three-step enzymatic pathway. The ubiquitin-activating enzyme (E1) plays a key role in the first step of ubiquitination pathway to activate ubiquitin or ubiquitin-like proteins. UBA5 had been proved to activate an ubiquitin-like protein, ubiquitin-fold modifier 1 (Ufm1), by forming a high-energy thioester bond. It localizes mainly in cytoplasm, while it mainly localizes to the nucleus in presence of SUMO2. Recombinant human UBA5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 47.4kDa (428aa) confirmed by MALDI-TOF
- NCBI登录号
- NP_079094
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