ZWINT Protein (AA 1-277) (His tag)
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- 抗原 See all ZWINT 蛋白
- ZWINT (ZW10 Interactor (ZWINT))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-277
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This ZWINT protein is labelled with His tag.
- 原理
- Recombinant Human ZW10 Interactor/ZWINT (N-6His)
- 序列
- MGSSHHHHHH SSGLVPRGSH MEAAETEAEA AALEVLAEVA GILEPVGLQE EAELPAKILV EFVVDSQKKD KLLCSQLQVA DFLQNILAQE DTAKGLDPLA SEDTSRQKAI AAKEQWKELK ATYREHVEAI KIGLTKALTQ MEEAQRKRTQ LREAFEQLQA KKQMAMEKRR AVQNQWQLQQ EKHLQHLAEV SAEVRERKTG TQQELDGVFQ KLGNLKQQAE QERDKLQRYQ TFLQLLYTLQ GKLLFPEAEA EAENLPDDKP QQPTRPQEQS TGDTMGRDPG VSFKAVGLQP AGDVNLP
- 产品特性
- Recombinant Human ZW10 Interactor/ZWINT is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Pro277) of Human ZWINT fused with a His tag at the N-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product ZWINT 蛋白
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ZW10 Interactor (ZWINT) (AA 1-277) protein (GST tag)
ZWINT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
ZW10 Interactor (ZWINT) (AA 1-230) protein (His tag)ZWINT 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
ZW10 Interactor (ZWINT) (Transcript Variant 3) protein (His tag)ZWINT 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
ZW10 Interactor (ZWINT) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)ZWINT 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
ZW10 Interactor (ZWINT) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)ZWINT 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - 有效期
- 3 months
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- 抗原
- ZWINT (ZW10 Interactor (ZWINT))
- 别名
- ZWINT (ZWINT 产品)
- 别名
- ZWINT Protein, HZwint-1 Protein, KNTC2AP Protein, ZWINT1 Protein, 2010007E07Rik Protein, 2600001N01Rik Protein, D10Ertd749e Protein, Zwint-1 Protein, Sip30 Protein, ZW10 interacting kinetochore protein Protein, ZW10 interactor Protein, ZWINT Protein, Zwint Protein
- Sub Type
- Fusionprotein
- 背景
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ZW10 Interactor is localized to the kinetochores from late Prophase to Anaphase and has a uniform distribution in the cytoplasm of Interphase cells. ZWINT interacts ZW10, MIS12 and NDC80 subunit of the NDC80 complex specifically during mitosis. ZWINT is a part of the MIS12 complex which is required for kinetochore formation and spindle checkpoint activity. In addition, ZWINT is required to target ZW10 to the kinetochore at prometaphase.
Alternative Names: ZW10 Interactor, ZW10-Interacting Protein 1, Zwint-1, ZWINT - 分子量
- 33.4 kDa
- UniProt
- O95229
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