GALE Protein (AA 1-348) (His tag)
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- 抗原 See all GALE 蛋白
- GALE (UDP-Galactose-4-Epimerase (GALE))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-348
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This GALE protein is labelled with His tag.
- 原理
- Recombinant Human UDP-Glucose 4-Epimerase/GALE (N-6His)
- 序列
- MGSSHHHHHH SSGLVPRGSH MAEKVLVTGG AGYIGSHTVL ELLEAGYLPV VIDNFHNAFR GGGSLPESLR RVQELTGRSV EFEEMDILDQ GALQRLFKKY SFMAVIHFAG LKAVGESVQK PLDYYRVNLT GTIQLLEIMK AHGVKNLVFS SSATVYGNPQ YLPLDEAHPT GGCTNPYGKS KFFIEEMIRD LCQADKTWNA VLLRYFNPTG AHASGCIGED PQGIPNNLMP YVSQVAIGRR EALNVFGNDY DTEDGTGVRD YIHVVDLAKG HIAALRKLKE QCGCRIYNLG TGTGYSVLQM VQAMEKASGK KIPYKVVARR EGDVAACYAN PSLAQEELGW TAALGLDRMC EDLWRWQKQN PSGFGTQA
- 产品特性
- Recombinant Human UDP-Glucose 4-Epimerase/GALE is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Ala348) of Human GALE fused with a His tag at the N-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product GALE 蛋白
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UDP-Galactose-4-Epimerase (GALE) (AA 1-348) protein (His tag)
GALE 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
UDP-Galactose-4-Epimerase (GALE) (AA 1-348) protein (GST tag)GALE 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
UDP-Galactose-4-Epimerase (GALE) (Transcript Variant 1) protein (His tag)GALE 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
UDP-Galactose-4-Epimerase (GALE) (AA 1-348), (full length) protein (His-SUMO Tag)GALE 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
UDP-Galactose-4-Epimerase (GALE) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)GALE 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
UDP-Galactose-4-Epimerase (GALE) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)GALE 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
UDP-Galactose-4-Epimerase (GALE) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)GALE 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Supplied as a 0.2 μm filtered solution of 50 mM TrisHCl, 150 mM NaCl, 2 mM DTT, 1 mM EDTA, pH 8.0.
- 储存液
- Dithiothreitol (DTT)
- 注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- -80 °C
- 储存方法
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - 有效期
- 6 months
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- 抗原
- GALE (UDP-Galactose-4-Epimerase (GALE))
- 别名
- UDP-Glucose 4-Epimerase/GALE (GALE 产品)
- 别名
- GALE Protein, im:7147391 Protein, wu:fb05f01 Protein, zgc:136578 Protein, F15H21.11 Protein, F15H21_11 Protein, REB1 Protein, ROOT EPIDERMAL BULGER1 Protein, ROOT HAIR DEFECTIVE 1 Protein, UDP-GLUCOSE 4-EPIMERASE Protein, UGE4 Protein, ECK0748 Protein, galD Protein, JW0742 Protein, SMU.888 Protein, BA5505 Protein, BA5700 Protein, VFA0352 Protein, galE Protein, 2310002A12Rik Protein, AI323962 Protein, 1n569 Protein, xgale Protein, SDR1E1 Protein, UDP-galactose-4-epimerase Protein, NAD(P)-binding Rossmann-fold superfamily protein Protein, UDP-galactose 4-epimerase GalE Protein, UDP-glucose 4-epimerase Protein, UDP-glucose 4-epimerase GalE Protein, UDP-glucose/UDP-N-acetylglucosamine 4-epimerase Protein, galactose-4-epimerase, UDP Protein, UDP-galactose-4-epimerase L homeolog Protein, GALE Protein, gale Protein, RHD1 Protein, ECs0787 Protein, galE Protein, galE1 Protein, galE2 Protein, STY0809 Protein, galE-2 Protein, SG0897 Protein, galD Protein, Ent638_1250 Protein, Gale Protein, gale.L Protein
- Sub Type
- Fusionprotein
- 背景
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The enzyme UDP-Glucose 4-Epimerase (GALE) is a homodimeric epimerase found in bacterial, plant and mammalian cells. UDP-Glucose 4-Epimerase performs the final step in the Leloir pathway of Galactose metabolism, it catalyzes two distinct but analogous reactions: the epimerization of UDP-Gglucose to UDP-Galactose and the epimerization of UDP-N-Acetylglucosamine to UDP-N-Acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids.
Alternative Names: UDP-Glucose 4-Epimerase, Galactowaldenase, UDP-Galactose 4-Epimerase, GALE - 分子量
- 40.44 kDa
- UniProt
- Q14376
- 途径
- Response to Water Deprivation, Cellular Glucan Metabolic Process
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