SPINK1 Protein (AA 24-79) (His tag)
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- 抗原 See all SPINK1 蛋白
- SPINK1 (serine Peptidase Inhibitor, Kazal Type 1 (SPINK1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 24-79
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宿主
- 人
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资源
- 人细胞
- 标记
- This SPINK1 protein is labelled with His tag.
- 原理
- Recombinant Human Serpin Kazal-1 (C-6His)
- 序列
- DSLGREAKCY NELNGCTKIY DPVCGTDGNT YPNECVLCFE NRKRQTSILI QKSGPCVDHH HHHH
- 产品特性
- Recombinant Human Serine Protease Inhibitor Kazal-Type 1/SPINK1 produced by transfected human cells is a secreted protein with sequence (Asp24-Cys79) of Human SPINK1 fused with a polyhistidine tag at the C-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product SPINK1 蛋白
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serine Peptidase Inhibitor, Kazal Type 1 (SPINK1) (AA 24-79) protein (His tag)
SPINK1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
serine Peptidase Inhibitor, Kazal Type 1 (SPINK1) (AA 1-79) protein (GST tag)SPINK1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
serine Peptidase Inhibitor, Kazal Type 1 (SPINK1) (AA 24-79) protein (GST tag)SPINK1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
serine Peptidase Inhibitor, Kazal Type 1 (SPINK1) (AA 24-79) protein (His tag)Crystallography grade SPINK1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
serine Peptidase Inhibitor, Kazal Type 1 (SPINK1) (AA 24-80) protein (His tag)Crystallography grade SPINK1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
serine Peptidase Inhibitor, Kazal Type 1 (SPINK1) proteinSPINK1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Supplied as a 0.2 μm filtered solution of 20 mM MES, 150 mM NaCl, 2 mM CaCl2, 1 mM DTT, 0.05 % Brij35, 10 % Glycerol, pH 6.0.
- 储存液
- Dithiothreitol (DTT)
- 注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- -80 °C
- 储存方法
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - 有效期
- 6 months
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- 抗原
- SPINK1 (serine Peptidase Inhibitor, Kazal Type 1 (SPINK1))
- 别名
- spink1 (SPINK1 产品)
- 别名
- PCTT Protein, PSTI Protein, Spink3 Protein, TATI Protein, TCP Protein, Psti Protein, SPINK1 Protein, p12 Protein, serine peptidase inhibitor, Kazal type 1 Protein, SPINK1 Protein, Spink1 Protein
- Sub Type
- Fusionprotein
- 背景
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Serine Protease Inhibitor Kazal-Type 1 (SPINK1) is a trypsin inhibitor that prevent the trypsin-catalyzed premature activation of zymogens within the pancreas. Defects in SPINK1 are a cause of pancreatitis (PCTT). A disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks. Defects in SPINK1 are the cause of susceptibility to tropical calcific pancreatitis (TCP). Recombinant SPINK1 protein (rSPINK1) stimulated cell proliferation in benign RWPE as well as cancerous prostate cells. The research result indicated that the potential of SPINK1 as an extracellular therapeutic target in prostate cancer. In contrast, knockdown of SPINK1 in 22RV1 cells inhibited cell proliferation, cell invasion, and tumor growth in xenograft assays.
Alternative Names: Pancreatic Secretory Trypsin Inhibitor, Serine Protease Inhibitor Kazal-Type 1, Tumor-Associated Trypsin Inhibitor, TATI, SPINK1, PSTI - 分子量
- 7.28 kDa
- UniProt
- P00995
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