Sonic Hedgehog Protein (SHH) (AA 24-197)
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北京 101111
Quick Overview for Sonic Hedgehog Protein (SHH) (AA 24-197) (ABIN1097559)
抗原
See all Sonic Hedgehog (SHH) 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 24-197
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原理
- Recombinant Human Sonic Hedgehog/SHH
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序列
- MCGPGRGFGK RRHPKKLTPL AYKQFIPNVA EKTLGASGRY EGKISRNSER FKELTPNYNP DIIFKDEENT GADRLMTQRC KDKLNALAIS VMNQWPGVKL RVTEGWDEDG HHSEESLHYE GRAVDITTSD RDRSKYGMLA RLAVEAGFDW VYYESKAHIH CSVKAENSVA AKSGG
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产品特性
- Recombinant Human Sonic Hedgehog is produced by our E.coli expression system and the target gene encoding Cys24-Gly197 is expressed.
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过滤
- 0.2 μm filtered
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内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Sonic Hedgehog (SHH) (AA 24-197), (Cys24IleIle-Mutant) (Active) protein
SHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Sonic Hedgehog (SHH) (AA 25-198), (Cys25IleIle-Mutant) (Active) proteinSHH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Sonic Hedgehog (SHH) (AA 181-280) protein (GST tag)SHH 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sonic Hedgehog (SHH) protein (His tag)SHH 宿主: 小鼠 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Sonic Hedgehog (SHH) (Active) protein (His tag)SHH 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
Sonic Hedgehog (SHH) protein (His tag)SHH 宿主: 人 宿主: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE. AM, ADA, IA, ScA
Sonic Hedgehog (SHH) (AA 25-198) protein (His tag)SHH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS
Sonic Hedgehog (SHH) (Active) proteinSHH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
Sonic Hedgehog (SHH) (AA 24-197) protein (His tag)SHH 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
Sonic Hedgehog (SHH) proteinSHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
缓冲液
- Lyophilized from a 0.2 um filtered solution of 20 mM PB, 100 mM NaCl, 1 mM DTT, pH 7.5.
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储存液
- Dithiothreitol (DTT)
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注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 3 weeks
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- Sonic Hedgehog (SHH)
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别名
- Sonic Hedgehog/SHH
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背景
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Alternative Names: Sonic Hedgehog Protein, SHH, HHG-1Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases. Human SHH is expressed as a 45kDa precursor, and undergoes a series of processing during secretion. After the removal of the signal peptide, a protease within the C-terminal domain catalyzes the cleavage of SHH into a 20 kDa N-terminal signaling domain (SHH-N) and a 25 kDa C-terminal domain (SHH-C). SHH-N has the “all signaling” capability. SHH-N binds to the 12 pass transmembrane protein Patched (Ptc) on cell surface, which releases the repression of the activity of Smoothened (Smo), a G-protein coupled receptor, by Ptc.
Alternative Names: Sonic Hedgehog Protein, SHH, HHG-1 -
分子量
- 19.69 kDa
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UniProt
- Q15465
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途径
- Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
抗原
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