POMGNT1 Protein (AA 1-660) (His tag)
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- 抗原 See all POMGNT1 蛋白
- POMGNT1 (Protein O-Linked Mannose Beta1,2-N-Acetylglucosaminyltransferase (POMGNT1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-660
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宿主
- 人
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资源
- 人细胞
- 标记
- This POMGNT1 protein is labelled with His tag.
- 原理
- Recombinant Human POMGNT1 (C-6His)
- 序列
- LDTRRAISEA NEDPEPEQDY DEALGRLEPP RRRGSGPRRV LDVEVYSSRS KVYVAVDGTT VLEDEAREQG RGIHVIVLNQ ATGHVMAKRV FDTYSPHEDE AMVLFLNMVA PGRVLICTVK DEGSFHLKDT AKALLRSLGS QAGPALGWRD TWAFVGRKGG PVFGEKHSKS PALSSWGDPV LLKTDVPLSS AVEAECHWAD TELNRRRRRF CSKVEGYGSV CSCKDPTPIE FSPDPLPDNK VLNVPVAVIA GNRPNYLYRM LRSLLSAQGV SPQMITVFID GYYEEPMDVV ALFGLRGIQH TPISIKNARV SQHYKASLTA TFNLFPEAKF AVVLEEDLDI AVDFFSFLSQ SIHLLEEDDS LYCISAWNDQ GYEHTAEDPA LLYRVETMPG LGWVLRRSLY KEELEPKWPT PEKLWDWDMW MRMPEQRRGR ECIIPDVSRS YHFGIVGLNM NGYFHEAYFK KHKFNTVPGV QLRNVDSLKK EAYEVEVHRL LSEAEVLDHS KNPCEDSFLP DTEGHTYVAF IRMEKDDDFT TWTQLAKCLH IWDLDVRGNH RGLWRLFRKK NHFLVVGVPA SPYSVKKPPS VTPIFLEPPP KEEGAPGAPE QTVDHHHHHH
- 产品特性
- Recombinant Human Protein O-Linked-Mannose beta-1 2-N-Acetylglucosaminyltransferase 1/POMGNT1 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Met1-Thr660) of Human POMGNT1 fused with a polyhistidine tag at the C-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product POMGNT1 蛋白
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Protein O-Linked Mannose Beta1,2-N-Acetylglucosaminyltransferase (POMGNT1) (AA 221-318) protein (GST tag)
POMGNT1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Protein O-Linked Mannose Beta1,2-N-Acetylglucosaminyltransferase (POMGNT1) (AA 1-660) protein (Strep Tag)Crystallography grade POMGNT1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Protein O-Linked Mannose Beta1,2-N-Acetylglucosaminyltransferase (POMGNT1) (AA 1-660) protein (Strep Tag)Crystallography grade POMGNT1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Protein O-Linked Mannose Beta1,2-N-Acetylglucosaminyltransferase (POMGNT1) (AA 59-660) protein (His tag)Crystallography grade POMGNT1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Protein O-Linked Mannose Beta1,2-N-Acetylglucosaminyltransferase (POMGNT1) (AA 59-660) protein (His tag)Crystallography grade POMGNT1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, 10 % Glycerol, pH 8.5.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- -80 °C
- 储存方法
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - 有效期
- 6 months
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- 抗原
- POMGNT1 (Protein O-Linked Mannose Beta1,2-N-Acetylglucosaminyltransferase (POMGNT1))
- 别名
- POMGNT1 (POMGNT1 产品)
- 别名
- POMGNT1 Protein, im:7151461 Protein, zgc:158130 Protein, 0610016I07Rik Protein, 4930467B06Rik Protein, GNTI.2 Protein, GnT I.2 Protein, LGMD2O Protein, MDDGA3 Protein, MDDGB3 Protein, MDDGC3 Protein, MEB Protein, MGAT1.2 Protein, gnT-I.2 Protein, protein O-linked mannose N-acetylglucosaminyltransferase 1 (beta 1,2-) Protein, protein O-linked mannose N-acetylglucosaminyltransferase 1 (beta 1,2-) S homeolog Protein, protein O-linked mannose beta 1,2-N-acetylglucosaminyltransferase Protein, POMGNT1 Protein, pomgnt1.S Protein, pomgnt1 Protein, Pomgnt1 Protein
- Sub Type
- Fusionprotein
- 背景
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Protein O-Linked-Mannose beta-1 2-N-Acetylglucosaminyltransferase 1 (POMGNT1) belongs to the Glycosyltransferase 13 family. Amino acid residues between 299-311 are important for both protein expression and enzymatic activity. The minimal catalytic domain is located between positions 299-651. It is suggested that the stem domain of the soluble form is unnecessary for activity, but that some amino acids play a crucial role in the membrane-bound form. Defects in POMGNT1 are the cause of muscular dystrophy-dystroglycanopathy congenital with brain and eye anomalies type A3 (MDDGA3).
Alternative Names: Protein O-Linked-Mannose Beta-1,2-N-Acetylglucosaminyltransferase 1, POMGnT1, UDP-GlcNAc:Alpha-D-Mannoside Beta-1,2-N-Acetylglucosaminyltransferase I.2, GnT I.2, POMGNT1, MGAT1.2 - 分子量
- 69.3 kDa
- UniProt
- Q8WZA1
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