Recombinant Human Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 2/LILRB2 produced by transfected human cells is a secreted protein with sequence (Gln22-His458) of Human LILRB2 fused with a polyhistidine tag at the C-terminus.
纯度
> 95 % as determined by reducing SDS-PAGE.
过滤
0.2 μm filtered
内毒素水平
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
Crystallography grade
LILRB2
宿主: 人
宿主: Insect Cells
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA, Crys
限制
仅限研究用
状态
Lyophilized
溶解方式
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
缓冲液
Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
注意事项
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
储存条件
4 °C/-20 °C/-80 °C
储存方法
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
有效期
3 months
抗原
LILRB2
(Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 2 (LILRB2))
CD85D Protein, ILT-4 Protein, ILT4 Protein, LIR-2 Protein, LIR2 Protein, MIR-10 Protein, MIR10 Protein, leukocyte immunoglobulin-like receptor subfamily A member 3 Protein, leukocyte immunoglobulin like receptor B2 Protein, LOC100433990 Protein, LILRB2 Protein
Sub Type
Fusionprotein
背景
Members of the immunoglobulin-like transcript (ILT) family are activating and inhibitory immunoreceptors whose genes are located same locus that encodes killer cell Ig-like receptors (KIR). Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 2 (LIR-2) is a type I transmembrane protein. LIR-2 is expressed primarily on monocytes and dendritic cells (DC). Human LIR-2 is produced as a 598 amino acino acid precursor including a 21 aa signal sequence, a 440 aa extracellular domain (ECD), a 21 aa transmenbrane segment, and a 116 aa cytoplasmic domain. LIR-2 binds to Classical MHCI proteins. Ligation of LIR-2 incluces Tyr phosphorylation within its cytoplasmic ITIMs, a requirement for association with SHP-1. LIR-2 mediates tolerogenic DC-induced CD4+ T cell energy in vitro and in vivo. Alternative Names: Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 2, LIR-2, Leukocyte Immunoglobulin-Like Receptor 2, CD85 Antigen-Like Family Member D, Immunoglobulin-Like Transcript 4, ILT-4, Monocyte/Macrophage Immunoglobulin-Like Receptor 10, MIR-10, CD85d, L