ASF1A Protein (AA 1-204) (His tag,T7 tag)
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- 抗原 See all ASF1A 蛋白
- ASF1A (ASF1 Anti-Silencing Function 1 Homolog A (S. Cerevisiae) (ASF1A))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-204
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This ASF1A protein is labelled with His tag,T7 tag.
- 原理
- Recombinant Human Histone Chaperone ASF1A/ASF1A (C-6His, N-T7 tag)
- 序列
- MASMTGGQQM GRGSMAKVQV NNVVVLDNPS PFYNPFQFEI TFECIEDLSE DLEWKIIYVG SAESEEYDQV LDSVLVGPVP AGRHMFVFQA DAPNPGLIPD ADAVGVTVVL ITCTYRGQEF IRVGYYVNNE YTETELRENP PVKPDFSKLQ RNILASNPRV TRFHINWEDN TEKLEDAESS NPNLQSLLST DALPSASKGW STSENSLNVM LESHMDCMLE HHHHHH
- 产品特性
- Recombinant Human Histone Chaperone ASF1A/ASF1A is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Met204) of Human ASF1A fused with a 6His tag at the C-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product ASF1A 蛋白
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ASF1 Anti-Silencing Function 1 Homolog A (S. Cerevisiae) (ASF1A) (AA 1-204) protein (GST tag)
ASF1A 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
ASF1 Anti-Silencing Function 1 Homolog A (S. Cerevisiae) (ASF1A) (AA 1-204) protein (Strep Tag)Crystallography grade ASF1A 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
ASF1 Anti-Silencing Function 1 Homolog A (S. Cerevisiae) (ASF1A) (AA 1-204) protein (Strep Tag)Crystallography grade ASF1A 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 1 mM DTT, 150 mM NaCl, pH 8.0.
- 储存液
- Dithiothreitol (DTT)
- 注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - 有效期
- 3 months
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- 抗原
- ASF1A (ASF1 Anti-Silencing Function 1 Homolog A (S. Cerevisiae) (ASF1A))
- 别名
- Histone Chaperone ASF1A/ASF1A (ASF1A 产品)
- 别名
- CGI-98 Protein, CIA Protein, HSPC146 Protein, 2310079C17Rik Protein, C79052 Protein, ASF1 Protein, ASF1A Protein, XAsf1 Protein, asf1 Protein, asf1-a Protein, asf1a Protein, asf1a-a Protein, asf1aa Protein, asf1ab Protein, anti-silencing function 1A histone chaperone Protein, anti-silencing function 1A histone chaperone L homeolog Protein, ASF1A Protein, Asf1a Protein, asf1a.L Protein
- Sub Type
- Fusionprotein
- 背景
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Human Histone Chaperone ASF1A (ASF1A) belongs to the H3/H4 family of histone chaperone proteins. ASF1A is ubiquitously expressed in many cells and tissues, interacting with histones H3 and H4. ASF1A cooperates with Chromatin Assembly Factor 1 to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. In addition, ASF1A is necessary for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Alternative Names: Histone Chaperone ASF1A, Anti-Silencing Function Protein 1 Homolog A, hAsf1, hAsf1a, CCG1-Interacting Factor A, CIA, hCIA, ASF1A - 分子量
- 25.4 kDa
- UniProt
- Q9Y294
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