Recombinant Human U6 snRNA-Associated Sm-Like Protein LSm4/LSM4 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Gln139) of Human LSM4 fused with a His tag at the N-terminus.
纯度
> 95 % as determined by reducing SDS-PAGE.
过滤
0.2 μm filtered
内毒素水平
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
缓冲液
Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 1 mM DTT, pH 8.0 .
储存液
Dithiothreitol (DTT)
注意事项
This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
储存条件
4 °C/-20 °C/-80 °C
储存方法
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
有效期
3 months
抗原
LSM4
(LSM4 Homolog, U6 Small Nuclear RNA Associated (LSM4))
别名
U6 SnRNA-Associated Sm-Like Protein LSm4 (LSM4 产品)
别名
GRP Protein, YER112W Protein, LSM4 homolog, U6 small nuclear RNA and mRNA degradation associated S homeolog Protein, LSM4 homolog, U6 small nuclear RNA and mRNA degradation associated Protein, lsm4.S Protein, LSM4 Protein, Lsm4 Protein
Sub Type
Fusionprotein
背景
U6 snRNA-associated Sm-like protein LSm4 (LSM4) is a member of the snRNP Sm proteins family. Sm-like proteins contain the Sm sequence motif and are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing. LSM4 forms a heteromer with a donut shape. The complexes are involved in various steps of RNA metabolism. LSM4 binds specifically to the 3-terminal U-tract of U6 snRNA. LSM4 contributes RNA protein interactions and structural changes which are essential during ribosomal subunit assembly. Alternative Names: U6 snRNA-Associated Sm-Like Protein LSm4, Glycine-Rich Protein, GRP, LSM4