LSM4 Protein (AA 1-139) (His tag)
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- 抗原 See all LSM4 蛋白
- LSM4 (LSM4 Homolog, U6 Small Nuclear RNA Associated (LSM4))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-139
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This LSM4 protein is labelled with His tag.
- 原理
- Recombinant Human U6 snRNA-Associated Sm-Like Protein LSm4/LSM4 (N-6His)
- 序列
- MGSSHHHHHH SSGLVPRGSH MLPLSLLKTA QNHPMLVELK NGETYNGHLV SCDNWMNINL REVICTSRDG DKFWRMPECY IRGSTIKYLR IPDEIIDMVK EEVVAKGRGR GGLQQQKQQK GRGMGGAGRG VFGGRGRGGI PGTGRGQPEK KPGRQAGKQ
- 产品特性
- Recombinant Human U6 snRNA-Associated Sm-Like Protein LSm4/LSM4 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Gln139) of Human LSM4 fused with a His tag at the N-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product LSM4 蛋白
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LSM4 Homolog, U6 Small Nuclear RNA Associated (LSM4) (AA 1-139) protein (His tag)
LSM4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
LSM4 Homolog, U6 Small Nuclear RNA Associated (LSM4) (AA 1-139) protein (GST tag)LSM4 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
LSM4 Homolog, U6 Small Nuclear RNA Associated (LSM4) (AA 1-73) protein (GST tag)LSM4 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
LSM4 Homolog, U6 Small Nuclear RNA Associated (LSM4) (AA 1-137) protein (Strep Tag)Crystallography grade LSM4 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
LSM4 Homolog, U6 Small Nuclear RNA Associated (LSM4) (AA 1-139), (full length) protein (His-SUMO Tag)LSM4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 1 mM DTT, pH 8.0 .
- 储存液
- Dithiothreitol (DTT)
- 注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - 有效期
- 3 months
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- 抗原
- LSM4 (LSM4 Homolog, U6 Small Nuclear RNA Associated (LSM4))
- 别名
- U6 SnRNA-Associated Sm-Like Protein LSm4 (LSM4 产品)
- 别名
- GRP Protein, YER112W Protein, LSM4 homolog, U6 small nuclear RNA and mRNA degradation associated S homeolog Protein, LSM4 homolog, U6 small nuclear RNA and mRNA degradation associated Protein, lsm4.S Protein, LSM4 Protein, Lsm4 Protein
- Sub Type
- Fusionprotein
- 背景
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U6 snRNA-associated Sm-like protein LSm4 (LSM4) is a member of the snRNP Sm proteins family. Sm-like proteins contain the Sm sequence motif and are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing. LSM4 forms a heteromer with a donut shape. The complexes are involved in various steps of RNA metabolism. LSM4 binds specifically to the 3-terminal U-tract of U6 snRNA. LSM4 contributes RNA protein interactions and structural changes which are essential during ribosomal subunit assembly.
Alternative Names: U6 snRNA-Associated Sm-Like Protein LSm4, Glycine-Rich Protein, GRP, LSM4 - 分子量
- 17.5 kDa
- UniProt
- Q9Y4Z0
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