GDNF Protein (AA 78-211)
Quick Overview for GDNF Protein (AA 78-211) (ABIN1096726)
抗原
See all GDNF 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 78-211
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原理
- Recombinant Human Glial Cell Line-Derived Neurotrophic Factor/GDNF
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序列
- SPDKQMAVLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCDAAETTYD KILKNLSRNR RLVSDKVGQA CCRPIAFDDD LSFLDDNLVY HILRKHSAKR CGCI
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产品特性
- Recombinant Human GDNF (Glial Cell Line-Derived Neurotrophic Factor) is produced by our E. coli expression system. The target protein is expressed with sequence (Ser78-Ile211) of Human GDNF.
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过滤
- 0.2 μm filtered
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内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 78-211) protein
GDNF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 78-211) protein (His tag)GDNF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 1-211) protein (GST tag)GDNF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 78-211) protein (Fc Tag)GDNF 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Glial Cell Line Derived Neurotrophic Factor (GDNF) proteinGDNF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 98,00% SDS
Glial Cell Line Derived Neurotrophic Factor (GDNF) proteinGDNF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 98,00% SDS
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.25.
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注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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储存条件
- 4 °C/-20 °C/-80 °C
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储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. -
有效期
- 3 months
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- GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))
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别名
- GDNF
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背景
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Recombinant Human GDNF: Glial Cell Line-Derived Neurotrophic Factor is a disulfide-linked homodimeric glycoprotein that belongs to the TGF-beta superfamily. It has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells. Mutations in this gene may be associated with Hirschsprung disease.
Alternative Names: Recombinant Human GDNF, Glial Cell Line-Derived Neurotrophic Factor, hGDNF, Astrocyte-Derived Trophic Factor, ATF, GDNF -
分子量
- 15.1 kDa
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UniProt
- P39905
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途径
- RTK signaling, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration
抗原
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