FCGR2A Protein (AA 34-219) (His tag)
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- 抗原 See all FCGR2A 蛋白
- FCGR2A (Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))
- 蛋白类型
- Recombinant
- 产品特性
- AA 34-219
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宿主
- 人
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资源
- 人细胞
- 标记
- This FCGR2A protein is labelled with His tag.
- 原理
- Recombinant Human Fc γ RIIa/FCGR2A/CD32a (C-6His)
- 序列
- APPKAVLKLE PPWINVLQED SVTLTCQGAR SPESDSIQWF HNGNLIPTHT QPSYRFKANN NDSGEYTCQT GQTSLSDPVH LTVLSEWLVL QTPHLEFQEG ETIMLRCHSW KDKPLVKVTF FQNGKSQKFS RLDPTFSIPQ ANHSHSGDYH CTGNIGYTLF SSKPVTITVQ VPSMGSSSPM GIIVDHHHHH H
- 产品特性
- Recombinant Human FcgammaRIIA/FCGR2A is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Gln34-Val219) of Human FCGR2A fused with a polyhistidine tag at the C-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product FCGR2A 蛋白
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Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 36-218) protein (His-Avi Tag)
FCGR2A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 28-208) protein (His tag)FCGR2A 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) protein (His-Avi Tag,Biotin)FCGR2A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 46-150) protein (GST tag)FCGR2A 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (Active) protein (His tag)FCGR2A 宿主: 人 宿主: CHO Cells Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (Active) protein (Fc Tag)FCGR2A 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by reducing SDS-PAGE. Active
Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (Active) protein (Biotin,His-Avi Tag)FCGR2A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 34-217) protein (His tag)Crystallography grade FCGR2A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 34-317) protein (rho-1D4 tag)Crystallography grade FCGR2A 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 36-218) (Active) protein (His tag)FCGR2A 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - 有效期
- 3 months
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- 抗原
- FCGR2A (Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))
- 别名
- cd32a (FCGR2A 产品)
- 别名
- CD32 Protein, CD32A Protein, CDw32 Protein, FCG2 Protein, FCGR2 Protein, FCGR2A1 Protein, FcGR Protein, IGFR2 Protein, CD32a Protein, Fc fragment of IgG receptor IIa Protein, FCGR2A Protein
- Sub Type
- Fusionprotein
- 背景
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Receptors for the Fc region of IgG (FcgammaR) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Human FcgammaRs are divided into three classes designated FcgammaRI (CD64), FcgammaRII (CD32), and FcgammaRIII (CD16), which generate multiple isoforms, are recognized. The activating- type receptor either has or associates non-covalently with an accessory subunit that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. FcgammaRI binds IgG with high affinity and functions during early immune responses, whereas FcgammaRII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Three genes for human FcgammaRII (A, B, and C) and one for mouse (FcgammaRIIB), encoding type I transmembrane proteins with ITAM motifs (FcgammaRII A and C) or ITIM motifs (FcgammaRIIB) in their cytoplasmic domains, have been identified. Human CD32, also known as Low affinity immunoglobulin gamma Fc region receptor II-a (IgG Fc receptor II-a), FcgammaRII A or FCGR2A Protein, is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Associated with an ITAM-bearing adapter subunit, FcRgamma, CD32a (FcgammaRII A) delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation, and cytokine production. The responses can be modulated by signals from the co-expressed inhibitory receptors such as Fcgamma RII B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.ReferencesKustiawan I, et al. Preventing adsorption of immunoglobulin G to solid surfaces using poloxamer 407 eliminates artifactual stimulation of neutrophils.PMID: 23545494 http://www.ncbi.nlm.nih.gov/pubmed/23545494
Alternative Names: Low Affinity Immunoglobulin Gamma Fc Region Receptor II-a, IgG Fc receptor II-a, CDw32, Fc-Gamma RII-a, Fc-Gamma-RIIa, FcRII-a, CD32, FCGR2A, CD32, FCG2, FCGR2A1, IGFR2 - 分子量
- 21.61kDa
- UniProt
- P12318
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