POLR2F Protein (AA 2-216, partial) (GST tag)
(2 references)-
- 抗原 See all POLR2F 蛋白
- POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-216, partial
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This POLR2F protein is labelled with GST tag.
- 应用范围
- ELISA
- 序列
- MLGTEGGEGF VVKVRGLPWS CSADEVQRFF SDCKIQNGAQ GIRFIYTREG RPSGEAFVEL ESEDEVKLAL KKDRETMGHR YVEVFKSNNV EMDWVLKHTG PNSPDTANDG FVRLRGLPFG CSKEEIVQFF SGLEIVPNGI TLPVDFQGRS TGEAFVQFAS QEIAEKALKK HKERIGHRYI EIFKSSRAEV RTHYDPPRKL MAMQRPGPYD RPGAG
- 产品特性
- Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.
- 纯度
- 90 %
- Top Product
- Discover our top product POLR2F 蛋白
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Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (AA 1-127) protein (His tag)
POLR2F 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (AA 1-127) protein (GST tag)POLR2F 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (AA 28-127) protein (GST tag)POLR2F 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (AA 2-127) protein (His tag)Crystallography grade POLR2F 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (AA 2-127) protein (His tag)Crystallography grade POLR2F 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) protein (Myc-DYKDDDDK Tag)POLR2F 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 说明
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The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- 0.2-2 mg/mL
- 缓冲液
- Tris-based buffer, 50 % glycerol
- 注意事项
- Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C for extended storage, conserve at -20 °C or -80 °C
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: "Genomic structure of the RNA polymerase II small subunit (hRPB14.4) locus (POLRF) and mapping to 22q13.1 by sequence identity." in: Genomics, Vol. 34, Issue 3, pp. 440-2, (1996) (PubMed).
: "A 14.4 KDa acidic subunit of human RNA polymerase II with a putative leucine-zipper." in: DNA sequence : the journal of DNA sequencing and mapping, Vol. 4, Issue 5, pp. 329-31, (1995) (PubMed).
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: "Genomic structure of the RNA polymerase II small subunit (hRPB14.4) locus (POLRF) and mapping to 22q13.1 by sequence identity." in: Genomics, Vol. 34, Issue 3, pp. 440-2, (1996) (PubMed).
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- 抗原
- POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))
- 别名
- DNA-directed RNA polymerases I, II, and III subunit RPABC2 protein (POLR2F 产品)
- 别名
- HRBP14.4 Protein, POLRF Protein, RPABC2 Protein, RPB14.4 Protein, RPB6 Protein, 1810060D16Rik Protein, RNA polymerase II subunit F Protein, polymerase (RNA) II (DNA directed) polypeptide F Protein, POLR2F Protein, Polr2f Protein
- 背景
- DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II, and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and togther with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds.
- 分子量
- 51.6 kD
- UniProt
- P31943
- 途径
- Regulatory RNA Pathways
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