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|抗原||V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) 抗体|
|抗原表位||AA 1-480 其他选择|
|适用||犬, Fly (Calliphora), 人, 小鼠, 大鼠 其他选择|
|标记||This AKT antibody is un-conjugated 其他选择|
Immunofluorescence (IF), Western Blotting (WB)
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AKT抗体详情AKT目标详情 使用细节 贮存及处理 References for anti-AKT antibody (ABIN968227) 图像
|交叉反应||犬, 小鼠, 大鼠, Fruit Fly (Drosophila melanogaster)|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|纯化方法||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|免疫原||Human PKBalpha/Akt1 aa. 1-480|
AKT目标详情AKT抗体详情 使用细节 贮存及处理 References for anti-AKT antibody (ABIN968227) 图像 回页首
|别名||Akt (AKT1 Antibody 摘要)|
Protein Kinase B (PKB), also known as Rac (Related to the A and C kinases) Protein Kinase, shows 73% and 68% to Protein Kinase C (PKC) and Protein Kinase A (PKA), respectively. It was also identified as the product of the v-akt oncogene from T-cell lymphomas. Akt/PKB mRNA has been reported to be expressed in a wide variety of cell lines and has been detected in porcine brain, heart, liver, kidney, muscle, and ovary. Rac phosphorylates a number of physiological substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. Akt/PKB is activated in response to growth factors through the activation of PI3-kinase and Ras. Activation of PI3-Kinase generates phosphatidylinositol 3,4-bisphosphate which may induce the membrane translocation of Akt/PKB coincident with its phosphorylation and activation. Cellular stress leading to the activation of the p38 MAP kinase also induces Akt/PKB activation, indicating a multiplicity of signaling pathways that activate Akt/PKB. Upon activation, Akt/PKB associates with some members of the PKC family of kinases, such as PKCdelta and PKCzeta. Downstream of Akt/PKB, GSK3 is thus far the only identified substrate for Akt/PKB phosphorylated in vivo. This antibody is routinely tested by western blot analysis.
Synonyms: PKBalpha, RAC-PK
|途径||PI3K-Akt Signaling, RTK signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling|
使用细节AKT抗体详情 AKT目标详情 贮存及处理 References for anti-AKT antibody (ABIN968227) 图像 回页首
Related Products: ABIN968551, ABIN967389
贮存及处理AKT抗体详情 AKT目标详情 使用细节 References for anti-AKT antibody (ABIN968227) 图像 回页首
|缓冲液||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|注意事项||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|储存方法||Store undiluted at -20° C.|
References for anti-AKT antibody (ABIN968227)AKT抗体详情 AKT目标详情 使用细节 贮存及处理 图像 回页首
Marte, Downward: "PKB/Akt: connecting phosphoinositide 3-kinase to cell survival and beyond." in: Trends in biochemical sciences, Vol. 22, Issue 9, pp. 355-8, 1997
Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, 1991
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