A synthetic peptide for use as a blocking control in assays to test for specificity of EIF2 S3 antibody, Alternative Names: EIF2S3 control peptide, EIF2S3 antibody Blocking Peptide, Anti-EIF2S3 Blocking Peptide, Eukaryotic Translation Initiation Factor 2 Subunit 3 Gamma 52Kda Blocking Peptide, EIF2 Blocking Peptide, EIF2G Blocking Peptide, EIF2gamma Blocking Peptide, EIF2S3, EIFS3-2, EIFS3 2, EIFS3-2 Blocking Peptide, EIFS3 2 Blocking Peptide
EIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.