When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
缓冲液
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
储存条件
-20 °C
储存方法
Shipped at ambient temperature, store at -20°C.
抗原
FCGR2B
(Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))
别名
CD32 Peptide, CD32B Peptide, FCG2 Peptide, FCGR2 Peptide, IGFR2 Peptide, FCGR2B Peptide, AI528646 Peptide, F630109E10Rik Peptide, Fc[g]RII Peptide, FcgRII Peptide, Fcgr2 Peptide, Fcgr2a Peptide, Fcr-2 Peptide, Fcr-3 Peptide, Ly-17 Peptide, Ly-m20 Peptide, LyM-1 Peptide, Lym-1 Peptide, FCRII Peptide, FcRII Peptide, Fc fragment of IgG receptor IIb Peptide, Fc fragment of IgG, low affinity IIb, receptor (CD32) Peptide, Fc receptor, IgG, low affinity IIb Peptide, low affinity immunoglobulin gamma Fc region receptor II Peptide, FCGR2B Peptide, Fcgr2b Peptide, LOC100856270 Peptide