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SH3 fusion protein pull downs using ITSN1SH3 A reveal diurnally varying binding of VGLUT1 with slightly reduced VGLUT1/dynamin ratios at the beginning of the light (ZT 0) or the dark (ZT 12) period. Phosphorylation increases binding of VGLUT1 but not of dynamin to EnphSH3. In contrast binding of dynamin to ITSN1SH3 A decreases under phosphorylating conditions with no changes in VGLUT1 binding.
component of Reelin signaling that acts predominantly by facilitating the VLDLR-Dab1 axis to direct neuronal migration in the cortex and hippocampus and to augment synaptic plasticity
Immunohistochemistry and histological and morphometric analyses revealed a twofold increase in proliferative endothelial cells and a 1.35-fold increase in proliferative alpha-smooth muscle actin-positive cells in the lungs of ITSN-deficient mice.
PRRT2 interacts with the Src homology 3 domain-bearing protein Intersectin 1, an intracellular protein involved in synaptic vesicle cycling.
Endocytic deficiency caused by ITSN knock-down modified Alk5 endocytic trafficking and enhances its degradation
It was concluded that miR-194 regulates ROMK channel activity by modulating ITSN1 expression thereby enhancing ITSN1/WNK-dependent endocytosis.
ITSN1 enhances Cbl activity, in part, by modulating the interaction of Cbl with Spry2 through recruitment of Shp2 phosphatase to the Cbl-Spry2 complex.
intersectin is an endocytic scaffold with a role in fast neurotransmitter release, which may be of prime importance for information processing in the brain
This study demonistrated that Itsn1 mutant mice showed severe deficits in Morris water maze and contextual fear memory tasks.
ITSN1 binds Pro-rich regions in both Cbl and Spry2, and interaction of ITSN1 with Spry2 disrupts Spry2-Cbl interaction, resulting in enhanced ubiquitylation of the EGFR.
Data show that intersectin-SH3D interacts directly with a small region of CdGAP highly enriched in basic residues and comprising a novel conserved xKx(K/R)K motif.
Swapping of SH3 domain binding partners might act as a switch controlling the actin nucleation activity of intersectin 1L.
Cdc42 and Ras cooperate to mediate cellular transformation by intersectin-L
Data suggest that PI3K-C2beta and AKT are epistatic to intersectin, and show that ITSN, independent of its role in endocytosis, regulates a critical cellular signaling pathway necessary for cell survival.
Itsn1 has a role in endocytosis in an in vivo mammalian model, and that a disruption in Itsn1 expression causes a disturbance in vesicle trafficking and endocytic function in the brain
WIP enhances the interaction of N-WASP with ITSN1 and promotes ITSN1/beta-actin association.
Here, we discover that intersectin-s binds DENND2B, a guanine nucleotide exchange factor for the exocytic GTPase Rab13, and this interaction promotes recycling of ligand-free EGFR to the cell surface. Our study thus reveals a novel mechanism controlling the fate of internalized EGFR with important implications for cancer.
study reports that vaccinia virus A36 contains three NPF motifs that interact with the Eps15 homology (EH) domains in intersectin-1 and Eps15 to promote the release of virus from infected cells and their subsequent spread
Silencing ITSN1 in neuroblastoma cells led to decreased tumor growth in an orthotopic mouse model. Orthotopic animal models can provide insight into the role of ITSN1 pathways in neuroblastoma tumorigenesis.
These findings provide the basis for further functional investigations of the ITSN/CR16 complex that may play an important role in actin remodeling and cellular invasion.
Abnormal expression of the intersectin1-L protein in epileptic brain tissue may play an important role in epilepsy, especially refractory epilepsy.
Study suggests critical roles of ITSN1-S in malignant glioma proliferation, indicating a potential usage of ITSN1-S in the therapeutic intervention as a novel molecular target
Our observations suggest that ITSN1 is an important general regulator of Cdc42-, Nck- and N-WASP-dependent actin polymerisation
ITSN1 and ITSN2 bind similar proline-rich ligands but are differentially recognized by SH2 domain-containing proteins.
Silencing ITSN1 significantly inhibits the anchorage independent growth of tumor cells in vitro.
the neuron-specific isoform of the stable tubule-only polypeptide (STOP) interacts with SH3A domain of ITSN1.
ITSN-1s, via its SH3A domain has the unique ability to regulate dyn2 assembly-disassembly and function during endocytosis
ITSN1 has two isoforms: ITSN1-long and ITSN1-short. siRNA-mediated down regulation of ITSN1-short inhibits migration and invasion of glioma cells.
study reveals a link between overexpression of specific ITSN1 isoforms and behavioral phenotypes
This novel mammalian ITSN1 isoform possesses a significantly altered domain structure and performs specific protein-protein interactions.
Downregulation of intersectin-1s induces apoptosis of brain glioblastoma cells.
Studied the inhibitory mechanism of ITSN1L, & id'd a novel short amino acid motif which mediates autoinhibition. Found this motif is located in the SH3 DH linker region, and show that W1221 acts as key residue in establishing inhibitory interaction.
These findings expand the role of ITSN1 as a scaffolding molecule bringing together components of endocytic complexes.
The identification of fifteen novel transcriptional isoforms of the human ITSN1 gene with full-length coding sequences , is reported.
microexons provide a mechanism for the control of tissue-specific interactions of ITSN1 and Src with their partners.
The protein encoded by this gene is a cytoplasmic membrane-associated protein that indirectly coordinates endocytic membrane traffic with the actin assembly machinery. In addition, the encoded protein may regulate the formation of clathrin-coated vesicles and could be involved in synaptic vesicle recycling. This protein has been shown to interact with dynamin, CDC42, SNAP23, SNAP25, SPIN90, EPS15, EPN1, EPN2, and STN2. Multiple transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been characterized so far.
, intersectin 1
, intersectin 1, isoform 1
, EH and SH3 domains protein 1
, Eh domain, SH3 domain regulator of endocytosis 1
, intersectin (SH3 domain protein 1A)
, intersectin short form 2 variant 2
, SH3 domain-containing protein 1A
, Src homology 3 domain-containing protein
, human intersectin-SH3 domain-containing protein SH3P17
, intersectin 1 short form variant 3
, intersectin 1 short form variant, 11
, intersectin short variant 12
, EH domain and SH3 domain regulator of endocytosis 1
, intersectin 1 (SH3 domain protein 1A)