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MCF-7 nuclear extract (H2O2 treated)

Nuclear Extract Cell Lysate MCF7 Cells H2O2 treated
产品编号 ABIN2669815
发货至: 中国
  • 资源
    人细胞
    产品特性

    Origin: human breast carcinoma

    组件
    2 x 100 μg of MCF-7 nuclear extract (H2O2 treated) at 2.50 μg/μl
    Lysed Cells
    MCF7 Cells
    Lysate Type
    Cell Lysate
    Lysate Fraction
    Nuclear Extract
    Lysate Treatment
    H2O2 treated
  • 限制
    仅限研究用
  • 浓度
    2.50 μg/μL
    缓冲液
    The MCF-7 nuclear extracts have been collected in Lysis Buffer after 3 hrs incubation with 0.2 mM H2O2. The Lysis Buffer consists of 20 mM Hepes ( pH 7.9), 100 mM KCl, 1 mM MgCl2, 20 % glycerol, 0.5 mM PMSF and 0.5 mM DTT. The protein content has been determined by a Bradford-based assay.
    储存液
    Dithiothreitol (DTT)
    注意事项
    This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    It ist recommended to aliquot the extracts into single-use fractions and then storing them at -80 °C. This eliminates repeated freeze/thaw cycles.
    储存条件
    -80 °C
    储存方法
    To ensure stability, extracts should be stored at -80°C.
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